去咖啡碱茶叶叶绿素提取及叶绿素铜钠盐制备  

Extraction of Chlorophyll from Decaffeinated Tea Leaves andPreparation of Sodium Copper Chlorophyllin

作　　者：张军 郑国义 丁松松 王伟立 ZHANG Jun;ZHENG Guoyi;DING Songsong;WANG Weili(Shandong Guangtongbao Pharmaceuticals Co.,Ltd.,Weifang 262500,China;Deshi Energy Technology Group Co.Ltd.,Dongying 257029,China;School of Chemistry and Materials Science,Ludong University,Yantai 264039,China)

机构地区：[1]山东广通宝医药有限公司,山东潍坊262500 [2]德仕能源科技集团股份有限公司,山东东营257029 [3]鲁东大学化学与材料科学学院,山东烟台264039

出　　处：《鲁东大学学报(自然科学版)》2025年第2期140-144,共5页Journal of Ludong University:Natural Science Edition

基　　金：山东省自然科学基金(ZR2022MB105)。

摘　　要：为了充分提取茶叶中的有效成分,本研究以提取完咖啡碱后的茶叶为原料,优化了提取叶绿素以及制备叶绿素铜钠盐的工艺条件。最终获得叶绿素提取以及叶绿素铜钠盐制备的最佳工艺条件如下。1)叶绿素提取:90%乙醇为提取溶剂,提取温度60℃,提取时间2 h,料液体积比1∶5。2)叶绿素铜钠盐制备:50℃条件下,10%氢氧化钠水溶液调整pH值至11,皂化40 min;10%硫酸铜水溶液,50℃下铜化1 h;10%氢氧化钠水溶液调整pH值至12,于50℃下成盐1 h。使用此方法制备的叶绿素铜钠盐质量指标符合GB 26406—2011标准。因此,提取完咖啡碱后的茶叶,可继续进行叶绿素提取,并且当茶叶中的叶绿素被提取完后,剩余茶叶残渣仍可应用到农业肥料、养殖饲料中,为连续生产提供了可能。In order to fully extract the active components in tea,this study optimized the technological conditions of extracting chlorophyll and preparing sodium copper chlorophyll from tea after extracting caffein.The optimum technological conditions for the final extraction of chlorophyll and the preparation of sodium copper chlorophyll are as follows.1)chlorophyll extraction:extract with 90%ethanol at 60℃for 2 h with solid-liquid ratio of 1∶5.2)preparation of sodium copper chlorophyllin:pH was adjusted to 11 using 10%NaOH,then saponification was carried out at 50℃for 40 min;then 10%CuSO 4 was added for copperization at 50℃within 1 h;pH was adjusted to 12 using 10%NaOH,to obtain the sodium salt at 50℃for 1h.The quality test indicates that the sodium copper chlorophyllin prepared by this method is consistent with the GB 26406—2011.Therefore,the tea leaves after extracting caffeine can continue to extract chlorophyll,and after the chlorophyll in the tea leaves is extracted,the remaining tea residue can still be used in agricultural fertilizers and breeding feeds,providing the possibility of continuous production.

关 键 词：去咖啡碱 茶叶 叶绿素 叶绿素铜钠盐 

分 类 号：O629.9[理学—有机化学]