灵芝孢子油对小鼠的抗疲劳作用  

Effect of Ganoderma lucidumspore oil on anti-fatigue property in mice

作　　者：谭满意 刘上[1] 吴媛妮 胡余明[1] TAN Manyi;LIU Shang;WU Yuanni;HU Yuming(Hunan Provincial Center for Disease Control and Prevention,Changsha,Hunan 410153,China)

机构地区：[1]湖南省疾病预防控制中心,湖南长沙410153

出　　处：《实用预防医学》2025年第3期330-333,共4页Practical Preventive Medicine

摘　　要：目的研究灵芝孢子油对小鼠的抗疲劳作用与机制。方法将SPF级雄性ICR小鼠随机分成对照组、低剂量组(0.20 g/kg·bw)、中剂量组(0.40 g/kg·bw)和高剂量组(0.60 g/kg·bw),连续灌胃30 d后,测定小鼠的负重游泳时间、生化指标、能量代谢指标和氧化应激指标,评价灵芝孢子油的抗疲劳作用。对小鼠的肝脏、肾脏和肌肉进行苏木精-伊红染色,评价灵芝孢子油对小鼠主要代谢器官的影响。结果与对照组比较,中剂量组负重游泳时间显著延长,丙二醛含量显著降低(P<0.05)。高剂量组腺苷三磷酸酶和超氧化物歧化酶活性显著升高,血乳酸和尿素氮含量显著降低(P<0.05)。中、高剂量组血糖、肝糖原、肌糖原水平、谷胱甘肽过氧化物酶活性显著升高,乳酸脱氢酶、肌酸激酶活性显著降低(P<0.05)。组织学观察发现各剂量组小鼠的肝、肾组织正常,肌肉组织的病理损伤减轻。结论灵芝孢子油具有一定的抗疲劳效果,其机制可能与增强运动耐力、改善机体能量代谢和提高机体抗氧化能力有关。Objective To study the anti-fatigue effect and mechanisms of Ganoderma lucidum(G.lucidum)spore oil on mice.Methods Specified-pathogen-free grade male ICR mice were randomly divided into the control group,the low-dose group(0.20 g/kg·bw),the medium-dose group(0.40 g/kg·bw)and the high-dose group(0.60 g/kg·bw).After the mice were orally administered for 30 days,the weight-bearing swimming time,biochemical indicators,energy metabolism indicators and oxidative stress indicators were measured to evaluate the anti-fatigue effect of G.lucidum spore oil.The effect of G.lucidum spore oil on major metabolic organs,namely liver,kidneys and muscle tissue,were evaluated with hematoxylin-eosin staining.Results Compared with the control group,the weight-bearing swimming time in the medium-dose group was significantly extended and the level of malondialdehyde was significantly reduced(P<0.05).The activities of ATPase and superoxide dismutase in the high-dose group increased significantly,but the levels of blood lactic acid and urea nitrogen decreased significantly(P<0.05).The levels of blood glucose,liver glycogen and muscle glycogen and the activity of glutathione peroxidase in the medium-dose group and the high-dose group increased significantly,while the activities of lactate dehydrogenase and creatine kinase decreased significantly(P<0.05).The histological study showed that liver and kidney tissue in each dose group was normal,and the extent of pathological injury of muscle tissue was alleviated.Conclusion G.lucidum spore oil has a certain anti-fatigue effect,and its mechanism may be related to enhancing exercise endurance,and improving energy metabolism and antioxidant capacity of the body.

关 键 词：灵芝孢子油 小鼠 抗疲劳 抗氧化 

分 类 号：R285.5[医药卫生—中药学]