N-氨甲酰谷氨酸对夏洛来种公羊血浆生化指标、精液品质及粪便微生物菌群结构的影响  

Effects of N-Carbamylglutamate on Plasma Biochemical Indicators,Semen Quality and Fecal Microbiota Structure of Charolais Breeding Rams

作　　者：常帅 栗铭谦 马桂霞 刘娜 张贺春 王春强 马巍 CHANG Shuai;LI Mingqian;MA Guixia;LIU Na;ZHANG Hechun;WANG Chunqiang;MA Wei(College of Animal Science and Veterinary Medicine,Jinzhou Medical University,Liaoning Jinzhou 121001,China;Chaoyang Chaomu Breeding Stock Farm Co.,Ltd.,Liaoning Chaoyang 122099,China)

机构地区：[1]锦州医科大学畜牧兽医学院,辽宁锦州121001 [2]辽宁省朝阳市朝牧种畜场有限公司,辽宁朝阳122099

出　　处：《饲料工业》2025年第8期79-87,共9页Feed Industry

基　　金：国家级大学生创新项目[202310160010];辽宁省大学生创新项目[S202310160033];辽宁省教育厅面上项目[JYTMS20231736];科技特派行动专项计划项目[2024JH5/10400038]。

摘　　要：试验旨在研究日粮中添加N-氨甲酰谷氨酸(N-carbamylglutamate, NCG)对夏洛来种公羊血浆生化指标、精液品质及粪便微生物菌群结构的影响。选择16头夏洛来种公羊,随机分为两组,对照组饲喂基础日粮,试验组在基础日粮中添加18 g/d NCG,试验期50 d,其中预饲期15 d,正试期35 d。结果表明:与对照组相比,试验组种公羊血浆中总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)分别增加4.46%、1.99%和6.29%(P>0.05),谷草转氨酶(AST)降低20.97%(P>0.05),谷丙转氨酶(ALT)、乳酸菌脱氢酶(LDH)、肌酸激酶(CK)分别降低28.38%、17.68%和33.33%(P<0.05);试验组种公羊精子活力增加40.48%(P<0.05),采精量和精子密度分别增加和降低了24.49%和4.25%(P>0.05);试验组放线菌门(Actinobacteriota)、欧陆森氏菌属(Olsenella)的相对丰度显著提高(P<0.05),埃希氏-志贺氏菌属(Escherichia-Shigella)、隐秘杆菌属(Trueperella)等肠道致病菌的相对丰度显著降低(P<0.05)。综上,日粮中添加NCG可在一定程度上改善夏洛来种公羊肝脏和心肌功能,提高种公羊的精子活力、粪便微生物菌群多样性和有益菌相对丰度。The aim of this experiment was to study the effects of adding N-carbamylglutamate(NCG)to the diet on plasma biochemical indices,semen quality and fecal microflora structure of the Charolais breeding rams.Sixteen Charolais rams were randomly divided into two groups(control group and treatment group),the control group were fed with basal diet,while the rams in treatment group were fed with 18 g/d NCG in the basal diet,and the experimental period was 50 days,with a pre-feeding period of 15 days and an official period of 35 days.The results showed the following:compared with the control group,the total protein(TP),albumin(ALB),and globulin(GLB)in the plasma of the treated group of breeding rams increased by 4.46%,1.99%,and 6.29%,respectively(P>0.05),while the aspartate aminotransferase(AST)decreased by 20.97%(P>0.05).The alanine aminotransferase(ALT),lac-tate dehydrogenase(LDH),and creatine kinase(CK)significantly decreased by 28.38%,17.68%,and 33.33%,respectively(P<0.05).The sperm motility of the treatment group significantly increased by 40.48%(P<0.05),while the sperm collection and sperm density increased and decreased by 24.49%and 4.25%,respectively(P>0.05).The relative abundance of Actinobacteria and Olsenella in the treatment group was significantly increased(P<0.05),while the relative abundance of intestinal pathogenic bacteria such as Escherichia-Shigella and Trueperella was significantly decreased(P<0.05).In summary,adding NCG to the diet can improve the liver and myocardial function of the Charolais breeding rams to a certain extent,enhance the sperm vitality,fecal microbial diversity,and relative abun-dance of beneficial bacteria.

关 键 词：N-氨甲酰谷氨酸 夏洛来种公羊 血浆生化指标 精液品质 粪便微生物菌群 

分 类 号：S816.7[农业科学—饲料科学]