响应面法优化驴源枯草芽孢杆菌发酵培养基及发酵条件  

Optimization of Fermentation Medium and Conditions of Bacillus subtilis from Donkey by Response Surface Methodology

作　　者：王欢 白长胜 尹珺伊 张军 刘秋瑾 田秋丰 王岩 江波涛 WANG Huan;BAI Changsheng;YIN Junyi;ZHANG Jun;LIU Qiujin;TIAN Qiufeng;WANG Yan;JIANG Botao(Branch of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences,Heilongjiang Qiqihar 161005,China;Heilongjiang Engineering Technology Research Center of Fermented with Chinese Herbs for Feed,Heilongjiang Qiqihar 161005,China)

机构地区：[1]黑龙江省农业科学院畜牧兽医分院,黑龙江齐齐哈尔161005 [2]黑龙江省饲用中草药发酵工程技术研究中心,黑龙江齐齐哈尔161005

出　　处：《饲料工业》2025年第8期132-141,共10页Feed Industry

基　　金：黑龙江省农业科学院科技创新跨越工程特色产业项目[CX23TS21];黑龙江省农业科学院畜牧兽医分院自拟项目[ZNKT-202214、CNYGG-2024015]。

摘　　要：研究以从新鲜驴粪中筛选出高产纤维素酶的枯草芽孢杆菌LV2为研究对象,优化其发酵产纤维素酶工艺。以单因素试验、响应面试验分析培养基营养成分(氮源、碳源、无机盐)和发酵条件(接种量、温度、时间、pH、转速)对纤维素酶活性的影响。结果显示,枯草芽孢杆菌LV2产纤维素酶的最佳发酵参数为:蔗糖54.4 g/L,酵母粉20.6 g/L,磷酸氢二钠5.2 g/L,接种量6%,培养温度37℃,培养时间24 h,发酵初始pH 7.0,转速200 r/min。在最佳发酵参数下,LV2经24 h发酵纤维素酶产量为59.19 U/mL,是优化前的3.08倍;枯草芽孢杆菌LV2在纤维素分泌和水解方面具有良好的应用前景。This study selected Bacillus subtilis LV2 with high cellulase production from fresh donkey dung as the research object,and optimized its fermentation technology for cellulase production.The effects of nutrient components of medium(nitrogen source,carbon source,inorganic salt)and fermentation conditions(inocula-tion amount,temperature,time,pH and rotational speed)on cellulase activity were analyzed by singlefactor testand response surface test.The results showed that the optimum fermentation parameters for cellulase pro-duction by Bacillus strains LV2 were as follows:sucrose 54.4 g/L,yeast powder 20.6 g/L,disodium hydrogen phosphate 5.2 g/L,inoculation amount 6%,culture temperature 37℃,culture time 24 h,initial pH 7.0,and optimal rotational speed 200 r/min.Under the opti-mal fermentation parameters,the cellulase yield of LV2 after 24 h fermentation was 59.19 U/mL,which was 3.08 times higher than that before opti-mization.Bacillus subtilis LV2 had a good applica-tion prospect in cellulose secretion and hydrolysis.

关 键 词：枯草芽孢杆菌 培养基优化 发酵条件优化 纤维素酶 响应面法 

分 类 号：S816[农业科学—饲料科学]