短果大叶芹根部水提取物的化学成分及其抗氧化效果  

Chemical Components and Antioxidant Effects of Water Extract from the Roots of Spuriopimpinella brachycarpa

作　　者：乔燕 韩君 袁强华[3] QIAO Yan;HAN Jun;YUAN Qianghua(Inner Mongolia Brain Hospital(Third Hospital),Hohhot 010010,China;Beijing Tcmages Pharmaceutical Co.,Ltd.,Beijing 101301,China;Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China)

机构地区：[1]内蒙古自治区脑科医院(第三医院),内蒙古呼和浩特010010 [2]北京康仁堂药业有限公司,北京101301 [3]成都中医药大学附属医院,四川成都610075

出　　处：《中国现代中药》2025年第4期667-673,共7页Modern Chinese Medicine

基　　金：成都中医药大学附属医院科研能力提升“百人计划”优秀人才项目(22-Y31)。

摘　　要：目的:研究短果大叶芹Spuriopimpinella brachycarpa(Kom.)Kitag.根部水提取物的抗氧化能力及其对秀丽隐杆线虫寿命的影响,探索其药用潜力。方法:使用超高效液相色谱串联四极杆静电场轨道阱质谱法(UHPLC-Q-Exactive Orbitrap MS)鉴定短果大叶芹根部水提取物中的化学成分。使用野生型和转基因线虫SOD-3::GFP评估短果大叶芹根部水提取物的抗氧化效果。以100 mmol·L^(–1)百草枯诱导氧化应激模型,采用不同质量浓度(125~500 mg·L^(–1))的提取物处理L1期线虫,评估其对线虫抗氧化酶活性、活性氧水平及寿命的影响。结果:化学分析发现,短果大叶芹根部水提取物含有绿原酸、咖啡酸和奎宁酸等酚酸类化合物,这些成分具有显著的抗氧化活性。提取物能显著减少线虫体内活性氧(ROS)的产生,使其恢复至正常水平,逆转氧化应激导致的SOD-3::GFP表达下降,从而维持超氧化物歧化酶(SOD)活性。短果大叶芹根部水提取物还能延缓百草枯诱导的线虫死亡,显示出良好的抗氧化效果和寿命延长作用。结论:短果大叶芹根部水提取物通过增强线虫的抗氧化防御系统,降低氧化应激,进而延长线虫寿命。这为其作为天然抗氧化剂的应用提供了科学依据。Objective:To study the antioxidant capacity of the water extract from the roots of Spuriopimpinella brachycarpa(Kom.)Kitag.and its effect on the lifespan of Caenorhabditis elegans,and to explore its medicinal potential.Methods:The chemical components in the water extract from the roots of S.brachycarpa were identified using ultra-high-performance liquid chromatography coupled with quadrupole Orbitrap mass spectrometry(UHPLC-Q-Exactive Orbitrap-MS).Wild-type and transgenic SOD-3::GFP C.elegans were used to evaluate the antioxidant effects of the water extract from S.brachycarpa roots.An oxidative stress model was induced by 100 mmol·L^(-1) paraquat,and different concentrations(125-500 mg·L^(-1))of the extract were administered to L1 stage C.elegans.The effects on antioxidant enzyme activity,reactive oxygen species(ROS)levels,and lifespan were evaluated.Results:Chemical analysis revealed that the water extract from the roots of S.brachycarpa contained phenolic acids such as chlorogenic acid,caffeic acid,and quinic acid,which exhibited significant antioxidant activity.The extract significantly reduced the production of ROS in the C.elegans,restoring them to normal levels,and rescued the decline in SOD-3::GFP expression caused by oxidative stress,thereby maintaining the activity of superoxide dismutase(SOD).The water extract also delayed the death of C.elegans induced by paraquat,showing strong antioxidant effects and prolonging lifespan.Conclusion:The water extract from the roots of S.brachycarpa enhances the antioxidant defense system of C.elegans,reduces oxidative stress,and prolongs lifespan,providing a scientific basis for its application as a natural antioxidant.

关 键 词：短果大叶芹 根部水提取物 抗氧化 秀丽隐杆线虫 寿命延长 酚酸类化合物 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]