Chromosome-level genome of the parthenogenetic booklouse Liposcelis bostrychophila reveals high heterozygosity and a nonhomologous chromosome  

作　　者：Shi-Die Jiang Yu-Ang Chen Peng-Yu Guo Jun Sun Hua-Yue Feng Yi Li Jin-Jun Wang Dan-Dan Wei 

机构地区：[1]Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University,Chongqing,China [2]Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River(Ministry of Education),Academy of Agricultural Sciences,Southwest University,Chongqing,China

出　　处：《Insect Science》2025年第2期437-456,共20页昆虫科学(英文版)

基　　金：supported by the National Natural Science Foundation of China(31972276 and 32272528);the Special Fund for Youth Team of the Southwest Universities(SWU-XJLJ202304).

摘　　要：Psocodean species are emerging as significant sanitary and stored-product pests,posing threats to human health and global food security.Out of an estimated 10000 species,the whole genome sequences of only 4 species have been published.Genomic resources are crucial for establishing effective pest control and enhancing our understanding of the evolution of psocodean species.In this study,we employed Illumina and PacBio sequencing along with Hi-C scaffolding techniques to generate a chromosome-level genome assembly for the parthenogenetic booklouse Liposcelis bostrychophila.The assembled genome of this booklouse measures 291.67 Mb in length and comprises 9 chromosomes.Notably,the genome of L.bostrychophila exhibits a high level of heterozygosity and features a distinctive nonhomologous chromosome.This heterozygous characteristic of the parthenogenetic booklouse genome may arise from high mutation rates,based on genomic variations analysis across multiple generations.Our analysis revealed significantly expanded gene families,primarily associated with the detoxification and feeding habits of L.bostrychophila.These include integument esterases(ESTs),ATP-binding cassette(ABC)transporter genes and gustatory receptors(GRs).The high-quality genome sequence of L.bostrychophila provides valuable resources for further study on the molecular mechanisms of stress resistance.It enables researchers to identify crucial functional genes and facilitates research on the population genetics,evolution and phylogeny of booklice.

关 键 词：CHROMOSOME detoxification genes gustatory receptors insecticide resistance psocids 

分 类 号：Q96[生物学—昆虫学]