Knockdown of vitellogenin receptor based on minute insect RNA interference methods affects the initial mature egg load in the pest natural enemy Trichogramma dendrolimi  

作　　者：Cheng-Xing Wang Hui-Qiao Bao Zhi-Chao Yan Jie Wang Su Wang Yuan-Xi Li 

机构地区：[1]Department of Entomology,College of Plant Protection,Nanjing Agricultural University,Nanjing,China [2]Institute of Plant Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing,China

出　　处：《Insect Science》2025年第2期487-500,共14页昆虫科学(英文版)

基　　金：supported by the National Natu-ral Science Foundation of China(32172483);Jiangsu Agriculture Science and Technology Innovation Fund(CX(22)3012);National Key R&D Plan of China(2023YFD1400605).

摘　　要：Vitellogenin receptor(VgR)plays a crucial role in oogenesis by mediating en-docytosis of vitellogenin and a portion of the yolk proteins in many insect species.How-ever,the function of VgR in minute parasitoid wasps is largely unknown.Here,we applied Trichogramma dendrolimi,a minute egg parasitoid,as a study model to investigate the function of VgR in parasitoids.We developed RNA interference(RNAi)methods based on microinjection of prepupae in T.dendrolimi.RNAi employs nanomaterial branched amphipathic peptide capsules(BAPC)as a carrier for double-stranded RNA(dsRNA),sig-nificantly enhancing delivery efficiency.Also,artificial hosts without medium were used to culture the injected prepupae in vitro.Utilizing these methods,we found that ovarian growth was disrupted after knockdown of TdVgR,as manifested by the suppressed devel-opment of the ovariole and the inhibition of nurse cell internalization by oocytes.Also,the initial mature egg load in the ovary was significantly reduced.Notably,the parasitic capacity of the female adult with ovarian dysplasia was significantly decreased,possibly resulting from the low availability of mature eggs.Moreover,ovarian dysplasia in T.den-drolimi caused by VgR deficiency are conserved despite feeding on different hosts.The results confirmed a critical role of TdVgR in the reproductive ability of T.dendrolimi and provided a reference for gene functional studies in minute insects.

关 键 词：initial mature egg load ovarian dysplasia parasitic capacity RNA interference vitellogenin receptor 

分 类 号：Q966[生物学—昆虫学]