Candidatus Liberibacter asiaticus influences the emergence of the Asian citrus psyllid Diaphorina citri by regulating key cuticular proteins  

作　　者：Chen-Yang Yuan Yi-Fan Gao Yi Liu Jia-Yao Fan Ying-Zhe Yuan Long Yi Tian-Xing Jing Wei Dou Jin-Jun Wang 

机构地区：[1]Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University,Chongqing,China [2]Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River(Ministry of Education),Academy of Agricultural Sciences,Southwest University,Chongqing,China [3]National Citrus Engineering Research Center,Citrus Research Institute,Southwest University,Chongqing,China [4]National Navel Orange Engineering Research Center,Gannan Normal University,Ganzhou,Jiangxi Province,China [5]College of Horticulture and Plant Protection,Yangzhou University,Yangzhou,Jiangsu Province,China

出　　处：《Insect Science》2025年第2期501-514,共14页昆虫科学(英文版)

基　　金：supported by the National Key R&D Program of China(2021YFD1400805);the National Natural Science Foundation of China(32160625);the Science and Technology Projects in Jiangxi Province(20225BCJ22005)and the China Agriculture Research System:CARS-26.

摘　　要：The Asian citrus psyllid,Diaphorina citri,is the primary vector of the HLB pathogen,Candidatus Liberibacter asiaticus(CLas).The acquisition of CLas shortens the developmental period of nymphs,accelerating the emergence into adulthood and thereby facilitating the spread of CLas.Cuticular proteins(CPs)are involved in insect emergence.In this study,we investigated the molecular mechanisms underlying CLas-promoted emergence in D.citri via CP mediation.Here,a total of 159 CP genes were first identified in the D.citri genome.Chromosomal location analysis revealed an uneven distribution of these CP genes across the 13 D.citri chromosomes.Proteomic analysis identified 54 differentially expressed CPs during D.citri emergence,with 14 CPs exhibiting significant differential expression after CLas acquisition.Five key genes,Dc18aa-1,Dc18aa-2,DcCPR-24,DcCPR-38 and DcCPR-58,were screened from the proteome and CLas acquisition.The silencing of these 5 genes through a modified feeding method significantly reduced the emergence rate and caused various abnormal phenotypes,indicating the crucial role that these genes play in D.citri emergence.This study provides a comprehensive overview of the role of CPs in D.citri and reveals that CLas can influence the emergence process of D.citri by regulating the expression of CPs.These key CPs may serve as potential targets for future research on controlling huanglongbing(HLB)transmission.

关 键 词：Candidatus Liberibacter asiaticus cuticular proteins Diaphorina citri EMERGENCE HUANGLONGBING 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]