The dynamics of N^(6)-methyladenine RNA modification in resistant and susceptible rice varieties responding to rice stem borer damage  

作　　者：Shuai Li Xin-Yang Tan Zhen He Chen Shen Ya-Li Li Lang Qin Chun-Qing Zhao Guang-Hua Luo Ji-Chao Fang Rui Ji 

机构地区：[1]Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Jiangsu Key Laboratory for Food and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology,Nanjing,China [2]College of Plant Protection,Nanjing Agricultural University,Nanjing,China [3]School of Plant Protection,Yangzhou University,Yangzhou,Jiangsu Province,China [4]Wuhan Benagen Technology Company Limited,Wuhan,China [5]Jiangsu Collaborative Innovation Center of Regional Modern Agriculture&Environmental Protection,Huaiyin Normal University,Huaian,Jiangsu Province,China [6]School of Life Sciences,Anhui Normal University/Key Laboratory for Conservation and Use of Important Biological Resources of Anhui Province,Wuhu,Anhui Province,China

出　　处：《Insect Science》2025年第2期530-550,共21页昆虫科学(英文版)

基　　金：supported by the National Natural Science Foundation of China(32302320,32272485);the Jiangsu Agricultural Science and Technology Independent Innovation Fund[CX(22)3018];the Earmarked Fund for China CARS-RICE(CARS-01).

摘　　要：N^(6)-methyladenosine(m^(6)A)is the most prevalent modification in cellular RNA which orchestrates diverse physiological and pathological processes during stress response.However,the differential m^(6)A modifications that cope with herbivore stress in resistant and susceptible crop varieties remain unclear.Here,we found that rice stem borer(RSB)larvae grew better on indica rice(e.g.,MH63,IR64,Nanjing 11)than on japonica rice varieties(e.g.,Nipponbare,Zhonghua 11,Xiushui 11).Then,transcriptome-wide m^(6)A profiling of representative resistant(Nipponbare)and susceptible(MH63)rice varieties were performed using a nanopore direct RNA sequencing approach,to reveal variety-specific m^(6)A modifications against RSB.Upon RSB infestation,m^(6)A methylation occurred in actively expressed genes in Nipponbare and MH63,but the number of methylation sites decreased across rice chromosomes.Integrative analysis showed that m^(6)A methylation levels were closely associated with transcriptional regulation.Genes involved in herbivorous resistance related to mitogen-activated protein kinase,jasmonic acid(JA),and terpenoid biosynthesis pathways,as well as JA-mediated trypsin protease inhibitors,were heavily methylated by m^(6)A,and their expression was more pronounced in RSB-infested Nipponbare than in RSB-infested MH63,which may have contributed to RSB resistance in Nipponbare.Therefore,dynamics of m^(6)A modifications act as the main regulatory strategy for expression of genes involved in plant–insect interactions,which is attributed to differential responses of resistant and susceptible rice varieties to RSB infestation.These findings could contribute to developing molecular breeding strategies for controlling herbivorous pests.

关 键 词：crop variety-specific m^(6)A N^(6)-methyladenosine plant-insect interactions RICE stem borer 

分 类 号：S435.11[农业科学—农业昆虫与害虫防治]