Functional analysis of SDR112C1 associated with fenpropathrin tolerance in Tetranychus cinnabarinus(Boisduval)  

作　　者：Jinhang Li Jialu Liu Lishu Peng Jingui Liu Lin Xu Junfeng He Longjiang Sun Guangmao Shen Lin He 

机构地区：[1]Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Southwest University,Chongqing,China [2]Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River,Ministry of Education,Chongqing,China [3]National Citrus Engineering Research Center,Southwest University,Chongqing,China [4]Key Scientific Research Base of Pest and Mold Control of Heritage Collection(Chongqing China Three Gorges Museum),State Administration of Cultural Heritage,Chongqing,China

出　　处：《Insect Science》2025年第2期585-599,共15页昆虫科学(英文版)

基　　金：funded by the National Natural Science Foundation of China(U2202202);the National Key Research,Development Program of China(2023YFD1400000);Chongqing China Three Gorges Museum independent project(3GM2022-KTZ06).

摘　　要：Short-chain dehydrogenases/reductases(SDRs)are ubiquitously distributed across diverse organisms and play pivotal roles in the growth,as well as endogenous and exogenous metabolism of various substances,including drugs.The expression levels of SDR genes are reportedly upregulated in the fenpropathrin(FEN)-resistant(FeR)strain of Tetranychus cinnabarinus.However,the functions of these SDR genes in acaricide tolerance remain elusive.In this study,the activity of SDRs was found to be significantly higher(2.26-fold)in the FeR strain compared to the susceptible strain(SS)of T.cinnabarinus.A specific upregulated SDR gene,named SDR112C1,exhibited significant overexpression(3.13-fold)in the FeR population compared with that in the SS population.Furthermore,the expression of SDR112C1 showed a significant increase in the response to FEN induction.Additionally,knockdown of the SDR112C1 gene resulted in decreased SDR activity and reduced mite viability against FEN.Importantly,heterologous expression and in vitro incubation assays confirmed that recombinant SDR112C1 could effectively deplete FEN.Moreover,the overexpression of the SDR112C1 gene in Drosophila melanogaster significantly decreased the toxicity of FEN to transgenic fruit flies.These findings suggest that the overexpression of SDR SDR112C1 is a crucial factor contributing to FEN tolerance in T.cinnabarinus.This discovery not only enhances our understanding of SDR-mediated acaricide tolerance but also introduces a new family of detoxification enzymes to consider in practice,beyond cytochrome P450s,carboxyl/choline esterases and glutathione S-transferases.

关 键 词：fenpropathrin tolerance heterologous expression polyphagous mite shortchain dehydrogenase transgenic fruit fly 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治]