miRNA-mediated insect-resistant transgenic rice poses no risk to a non-target parasitoid,Cotesia chilonis,via direct feeding or through its target host  

作　　者：Cong Dang Shan Xiao Fang Wang Qi Fang Hongwei Yao Kang He Fei Li Dawei Xue Gongyin Ye 

机构地区：[1]College of Life and Environmental Sciences,Hangzhou Normal University,Hangzhou,China [2]National Key Laboratory of Rice Biology and Breeding,Institute of Insect Sciences,Zhejiang University,Hangzhou,China [3]Ningbo Academy of Agricultural Sciences,Ningbo,Zhejiang Province,China

出　　处：《Insect Science》2025年第2期621-630,共10页昆虫科学(英文版)

基　　金：funded by Science and Technology Innovation 2030-"Agricultural Biological Breeding"Major Project(2023ZD04062);the Experimental Technology Research Program of Zhejiang University(SYB202126).

摘　　要：MicroRNAs(miRNAs)have started to play an important role in pest control,and novel miRNA-based transgenic insect-resistant plants are now emerging.However,an environmental risk assessment of these novel transgenic plants expressing insect miRNAs must be undertaken before promoting their application.Here,transgenic miR-14 rice,which has high resistance to the rice stem borer Chilo suppressalis,was used as an example for evaluation in this study.Taking the tier 1 risk assessment method in Bacillus thuringiensis(Bt)crops as a reference,the effects of the direct exposure of a non-target parasitoid,Cotesia chilonis,to a high concentration of miRNA were evaluated.The results showed that direct feeding with miR-14 at high concentration had no significant effects on the biological parameters of Co.chilonis,whereas when miR-14 was injected into Ch.suppressalis-parasitized larvae,the development duration of Co.chilonis was significantly affected.In combination with the real conditions of the rice paddy field,it could be inferred that transgenic miR-14 rice has no significant negative effects on the important non-target parasitoid,Co.chilonis.These results will provide a foundation for the establishment of a new safety evaluation system for novel RNAi-based transgenic plants.

关 键 词：Chilo suppressalis Cotesia chilonis miR-14 non-target effects rice 

分 类 号：S435.112[农业科学—农业昆虫与害虫防治]