RNAi-mediated pest control targeting the Troponin I(wupA)gene in sweet potato weevil,Cylas formicarius  

作　　者：Mengjun Zhang Xiaxuan Zhang Tingting Chen Yonglin Liao Bin Yang Guirong Wang 

机构地区：[1]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing,China [2]Shenzhen Branch,Guangdong Laboratory for Lingnan Modern Agriculture,Synthetic Biology Laboratory of the Ministry of Agriculture and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong Province,China [3]Institute of Plant Protection,Guangdong Academy of Agricultural Science,GuangdongProvincial Key Laboratory High Technology for Plant Protection,Guangzhou,China

出　　处：《Insect Science》2025年第2期631-648,共18页昆虫科学(英文版)

基　　金：supported from the National Key R&D Program of China(2022YFE0116500);the Shen zhen Science and Technology Program(Grant No.KQTD20180411143628272)and Agricultural Science and Technology Innovation Program(ASTIP).

摘　　要：The sweet potato weevil(Cylas formicarius)is a critical pest producing enor-mous global losses in sweet potato crops.Traditional pest management approaches for sweet potato weevil,primarily using chemical pesticides,causes pollution,food safety is-sues,and harming natural enemies.While RNA interference(RNAi)is a promising envi-ronmentally friendly approach to pest control,its efficacy in controlling the sweet potato weevil has not been extensively studied.In this study,we selected a potential target for controlling C.formicarius,the Troponin I gene(wupA),which is essential for muscula-ture composition and crucial for fundamental life activities.We determined that wupA is abundantly expressed throughout all developmental stages of the sweet potato weevil.We evaluated the efficiency of double-stranded RNAs in silencing the wupA gene via microinjection and oral feeding of sweet potato weevil larvae at different ages.Our find-ings demonstrate that both approaches significantly reduced the expression of wupA and produced high mortality.Moreover,the 1st instar larvae administered dswupA exhibited significant growth inhibition.We assessed the toxicity of dswupA on the no-target insect silkworm and assessed its safety.Our study indicates that wupA knockdown can inhibit the growth and development of C.formicarius and offer a potential target gene for envi-ronmentally friendly control.

关 键 词：BIOPESTICIDES gene knockdown IPM pest management RNA interference wupA 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]