基于影像转录组学颈痛慢性化的中枢跨尺度机制研究  

Investigation of central cross-scale mechanisms in the chronification of neck pain via imaging transcriptomics

作　　者：高珍 崔梦洁 王海军 冀来喜 GAO Zhen;CUI Mengjie;WANG Haijun;JI Laixi(Experimental Management Center,Shanxi University of Chinese Medicine,Jinzhong 030619,China;Traditional Chinese Medicine and Rehabilitation Department,Taiyuan Peace Hospital,Taiyuan 030024,China;The Second Clinical College,Shanxi University of Chinese Medicine,Jinzhong 030619,China)

机构地区：[1]山西中医药大学实验管理中心,晋中030619 [2]太原市和平医院中医康复科,太原030024 [3]山西中医药大学第二临床学院,晋中030619

出　　处：《磁共振成像》2025年第3期18-23,共6页Chinese Journal of Magnetic Resonance Imaging

基　　金：山西省基础研究计划项目(编号:202203021221195);山西中医药大学博士科研启动基金项目(编号:2023BK17);优秀博士毕业生来晋工作奖励经费科研启动基金项目(编号:2023BKS12)。

摘　　要：目的基于影像转录组学探讨急性颈痛向慢性颈痛转变的中枢病理机制。材料与方法2023年3月至2024年5月于山西中医药大学、山西省针灸医院招募急性颈痛患者86例,慢性颈痛患者89例。采用3.0 T MR扫描仪采集受试者基于血氧水平依赖性的静息态功能磁共振成像(resting-state functional magnetic resonance imaging,rs-fMRI)数据,以下丘脑为种子点进行全脑功能连接(functional connectivity,FC)分析;同时,利用艾伦人脑图谱(Allen Human Brain Atlas,AHBA)转录组数据集进行影像转录组分析,采用偏最小二乘(partial least squares,PLS)回归探讨慢性颈痛与急性颈痛患者大脑功能活动和基因表达的区域变化,并通过Metascape分析进行基因富集分析,以揭示颈痛慢性化过程中的中枢跨尺度机制。结果与急性颈痛相比,慢性颈痛患者存在左侧下丘脑和右侧眶部额上回FC值的增高,和右侧额中回FC值的降低(体素水平P<0.01,簇水平P<0.05)。此外,本研究发现PLS1模型解释了43.43%的方差,且PLS1加权基因表达图谱与病例对照t图空间正相关(皮尔逊r=0.491,P<0.05)。富集分析显示,PLS1+基因与“谷氨酸能突触”等细胞组成和“突触信号传导”等生物过程,PLS1-基因与“中间丝细胞骨架”等细胞组成、“DNA结合转录激活活性”等分子功能和“生长激素分泌的调节”等生物过程密切相关。结论基于影像转录组学的跨尺度分析揭示了颈痛慢性化过程中大脑功能活动、基因表达和细胞组成的综合变化,提示颈痛慢性化是一个涉及从分子层面到细胞结构,再到脑网络功能的多层次、多尺度相互作用的过程。Objective:To explore the central pathological mechanisms underlying the transition from acute to chronic neck pain using imaging transcriptomics.Materials and Methods:From March 2023 to May 2024,86 patients with acute neck pain and 89 patients with chronic neck pain were recruited from Shanxi University of Chinese Medicine and Shanxi Provincial Hospital of Acupuncture and Moxibustion.Using a 3.0 T MR scanner to collect resting-state functional magnetic resonance imaging(rs-fMRI) data based on blood oxygen level-dependent signals from the subjects,with the hypothalamus as the seed region for whole-brain functional connectivity(FC)analysis.Imaging transcriptomics analysis was performed using the Allen Human Brain Atlas(AHBA) transcriptomic dataset,and partial least squares(PLS) regression was employed to investigate regional changes in brain functional activity and gene expression between chronic and acute pain patients.Gene enrichment analysis was conducted using Metascape to reveal the central cross-scale mechanisms during the chronicification of neck pain.Results:Compared to acute neck pain,patients with chronic neck pain exhibited increased FC values in the left hypothalamus and right orbital superior frontal gyrus,and decreased FC values in the left hypothalamus and right middle frontal gyrus(Voxel-level P < 0.01,Cluster-level P < 0.05).Additionally,this study found that the PLS1 model explained 43.43%of the variance,and the PLS1weighted gene expression profile was positively correlated with the case-control t-map space(Pearson's r =0.491,P < 0.05).Enrichment analysis revealed that PLS1+ genes were closely associated with cellular components such as "glutamatergic synapse" and biological processes like "synaptic signaling," while PLS1-genes were closely related to cellular components like "intermediate filament cytoskeleton," molecular functions such as "DNA binding transcription activator activity," and biological processes including "regulation of growth hormone secretion".Conclusions:A cross-scale analysis

关 键 词：颈痛 影像转录组 功能连接 疼痛慢性化 磁共振成像 跨尺度机制 

分 类 号：R445.2[医药卫生—影像医学与核医学] R653[医药卫生—诊断学]