Platelet rich plasma,adipose tissue micrografts,and regenerative mimetic factors for abdominal wall defect reconstruction:Experimental study protocol  

作　　者：Konstantinos Zapsalis Orestis Ioannidis Christos Xylas Konstantinos Siozos Georgios Gemousakakis Elissavet Anestiadou Savvas Symeonidis Stefanos Bitsianis Efstathios Kotidis Angeliki Cheva Chryssa Bekiari Antonia Loukousia Konstantinos Angelopoulos Manousos-Georgios Pramateftakis Ioannis Mantzoros Freiderikos Tserkezidis Barbara Driagka Stamatios Angelopoulos 

机构地区：[1]Fourth Department of Surgery,Medical School,Aristotle University of Thessaloniki,Thessaloniki 57010,KentrikíMakedonía,Greece [2]Department of Pathology,Medical School,Aristotle University of Thessaloniki,Thessaloniki 54636,KentrikíMakedonía,Greece [3]Laboratory of Anatomy and Histology,School of Veterinary Medicine,Faculty of Health Sciences,Aristotle University of Thessaloniki,Thessaloniki 54124,KentrikíMakedonía,Greece

出　　处：《World Journal of Experimental Medicine》2025年第2期121-129,共9页世界实验医学杂志(英文)

摘　　要：BACKGROUND Incisional hernias are a common complication of previous surgeries and remain a persistent issue in clinical practice,posing a significant burden on healthcare systems despite advances in education and technology.Surgical techniques,primarily involving the use of mesh to cover the abdominal wall gap,are widely used as a standard intervention strategy.AIM To examine the regeneration of the aponeurosis defect in the anterior abdominal wall in rats using regenerative mimetic factors of the extracellular matrix[ReGeneraTing Agent(RGTA)],adipose tissue micrografts(ATM),and platelet rich plasma(PRP)as regenerative agents.METHODS Regenerative agents such as RGTA,ATM,and PRP are gaining popularity.ATM involves autologous adipose tissue cells with mesenchymal stem cell markers and a high percentage of stromal vascular fraction cells.RGTAs are heparan sulfate(HS)mimetics that replace degraded HSs in damaged tissue,enhancing the quality and speed of repair.PRP is a concentrated plasma preparation containing seven fundamental proteins responsible for tissue production.An acellular dermal matrix is a biological implant free of cellular or antigenic components,making it an excellent material for reconstructive surgery.Polyglactin is a synthetic,absorbable mesh that loses 50%of its strength after fourteen days,providing initial support for new tissue regeneration before being completely absorbed.RESULTS Rats will undergo a laparotomy with a precise 2 cm by 2 cm excision of the anterior abdominal wall fascia below the umbilicus.They will be divided into sixteen groups,each receiving different combinations of regenerative factor injections into the denervated area in both non-contaminated and contaminated environments.A collagenelastin matrix will be used to join the aponeurosis edges,with an absorbable polyglactin mesh anchored over it.Samples will be taken for macroscopic,histological,and immunohistochemical evaluation of tissue regeneration.CONCLUSION Our study aims to demonstrate how these factors promote cell prol

关 键 词：Incisional hernia Regenerative agents Platelet rich plasma Adipose tissue micrografts Regenerative mimetic factors Acellular collagen-elastin matrix Polyglactin mesh Abdominal wall defects LAPAROTOMY 

分 类 号：R656[医药卫生—外科学]