人参多肽温敏水凝胶对大鼠热源性皮肤损伤的修复作用及其机制  

Repair effect of ginseng polypeptide thermosensitive hydrogel on heat-induced skin injury in rats and its mechanism

作　　者：江俊杰 武皓 何康 伞志强 杨擎 李辉[3] 李娜 JIANG Junjie;WU Hao;HE Kang;SAN Zhiqiang;YANG Qing;LI Hui;LI Na(Ginseng Research Institute of Jilin Province,Changchun University of Chinese Medicine,Changchun 130017,China;Department of Rehabilitation,School of Nursing,Jilin University,Changchun 130021,China;Department of Surgery,Jilin Qianwei Hospital,Changchun 130012,China)

机构地区：[1]长春中医药大学吉林省人参科学研究院,吉林长春130017 [2]吉林大学护理学院康复治疗学教研室,吉林长春130021 [3]吉林省前卫医院外科,吉林长春130012

出　　处：《吉林大学学报(医学版)》2025年第2期360-369,共10页Journal of Jilin University:Medicine Edition

基　　金：吉林省科技厅科技发展计划项目(20210204086YY)。

摘　　要：目的:制备新型人参多肽温敏水凝胶,观察其对大鼠热源性皮肤损伤的修复作用并探讨其机制。方法:使用Pluronic F127和β-甘油磷酸钠(β-GP)制备温敏水凝胶,评估其相变温度、空间结构、元素组成和保水能力。建立大鼠热源性皮肤损伤模型,分为PBS组、凝胶(Gel)组和人参多肽凝胶(GP-Gel)组。分别处理大鼠创面11 d后,采用HE和Masson染色观察各组大鼠创面形态学改变和胶原沉积情况。免疫组织化学法检测各组大鼠皮肤创面组织中平滑肌肌动蛋白(α-SMA)、结缔组织生长因子(CTGF)、碱性成纤维细胞生长因子(bFGF)、增殖细胞核抗原(PCNA)、细胞增殖标志物Ki67、表皮生长因子(EGF)、CD31、血管内皮生长因子(VEGF)、P50和P65蛋白表达水平,Western blotting法检测各组大鼠皮肤创面中Toll样受体4 (TLR4)蛋白表达水平,ELISA法检测各组大鼠血清中肿瘤坏死因子α (TNF-α)、白细胞介素1β (IL-1β)、白细胞介素6 (IL-6)、白细胞介素15 (IL-15)和白细胞介素10 (IL-10)水平。结果:与PBS组和Gel组比较,GP-Gel组大鼠创面减小(P<0.01),皮肤组织中PCNA、Ki67、EGF、CD31、VEGF、α-SMA和CTGF蛋白表达水平增加(P<0.05或P<0.01),P65和TLR4蛋白表达水平降低(P<0.01),血清中抗炎因子IL-10水平增加(P<0.01),促炎因子TNF-α、 IL-1β、IL-6和IL-15水平降低(P<0.05或P<0.01)。结论:人参多肽温敏水凝胶通过促进细胞增殖、纤维增生、血管生成及减少炎症反应,促进热源性皮肤损伤的修复。Objective:To prepare a novel ginseng polypeptide thermosensitive hydrogel,and to investigate its repair effect on heat-induced skin injury in the rats and explore the underlying mechanisms.Methods:Thermosensitive hydrogels were formulated using Pluronic F127 andβ-sodium glycerophosphate(β-GP),and their phase transition temperatures,spatial structures,elemental compositions,and water retention capacities were evaluated.The rat models of heat-induced skin injury were established and the model rats were divided into PBS group,Gel group,and ginseng polypeptide gel(GP-Gel)group.After 11 d of treatment,the morphological changes of wound and collagen deposition in the wound of the rats in various groups were observed by HE and Masson staining.Immunohistochemistry was used to detect the expression levels ofα-smooth muscle actin(α-SMA),connective tissue growth factor(CTGF),basic fibroblast growth factor(bFGF),proliferating cell nuclear antigen(PCNA),cell proliferation marker Ki67,epidermal growth factor(EGF),CD31,vascular endothelial growth factor(VEGF),P50 and P65 proteins in the skin wound tissue of the rats in various groups.Western blotting method was used to detect the expression levels of Toll-like receptor 4(TLR4)in the skin wound tissue of the rats in various groups.ELISA method was used to measure the levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-15(IL-15),and interleukin-10(IL-10)in the serum of the rats in various groups.Results:Compared with PBS and Gel groups,the wound area of the rats in GP-Gel group was reduced(P<0.01),the expression levels of PCNA,Ki67,EGF,CD31,VEGF,α-SMA,and CTGF proteins in the skin wound tissue were increased(P<0.05 or P<0.01),and the expression levels of P65 and TLR4 proteins were decreased(P<0.01);the level of anti-inflammatory factor IL-10 in serum was increased(P<0.01),while the levels of pro-inflammatory factors TNF-α,IL-1β,IL-6 and IL-15 were decreased(P<0.05 or P<0.01).Conclusion:The ginseng polypeptide thermosensitive h

关 键 词：人参多肽 水凝胶 热源性皮肤损伤 炎症 血管生成 

分 类 号：R932[医药卫生—生药学]