利用谷氨酸棒杆菌产脂肪酸的初步研究  

Preliminary study on fatty acid production from Corynebacterium glutamicum

作　　者：潘清华 陈曦 周华[1] 蔡恒[1] PAN Qinghua;CHEN Xi;ZHOU Hua;CAI Heng(College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211800,China)

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211800

出　　处：《生物加工过程》2025年第2期160-167,共8页Chinese Journal of Bioprocess Engineering

摘　　要：以谷氨酸棒杆菌Corynebacterium glutamicum C.gNCF(ΔldhA::pyc,Δpqo::gapA,Δcat::pntAB,ΔfasR)为研究对象,利用pK18mobsacB自杀性质粒构建fadD5基因敲除菌NC e1,在此基础上,进一步敲除fasB,获得重组菌NC e2,然后考察NC e1与NC e2发酵产脂肪酸的能力。结果表明:菌株NC e1与NC e2的主要发酵产物为棕榈酸、油酸与硬脂酸。与原始菌C.gNCF的总脂肪酸产量212.2 mg/L相比,NC e1的总脂肪酸产量增加了29.5%,其中棕榈酸与油酸产量分别提高40%与21%;与NC e1相比,NC e2的油酸产量又提高16.9%,同时NC e2的油酸产量为原始菌C.gNCF的1.358倍。由此可见,fadD5和fasB是谷氨酸棒杆菌产脂肪酸路径中的关键基因,敲除fadD5有利于棕榈酸与油酸的积累,而敲除fasB有利于油酸的生成,通过同时敲除这2种基因可以实现油酸的增产。Two recombinant strains,NC-e1 and NC-e2,were produced from the modification of Corynebacterium glutamicum C.gNCF(ΔldhA::pyc,Δpqo::gapA,Δcat::pntAB,ΔfasR).The former was obtained through the knockout of fadD 5 by pK18mobsacB suicide plasmid,and the latter was generated through further knockout of fasB from NC-e1.Subsequently,fatty acid production was evaluated using NC-e1 and NC-e2 fermentation.As a result,NC-e1 and NC-e2 mainly produced palmitic acid,oleic acid and stearic acid.Compared with the total fatty acid yield(212.2 mg/L)from C.gNCF,NC-e1 led to 29.5%higher total fatty acid,with palmitic acid and oleic acid yield increasing by 40%and 21%,respectively.In addition,NC-e2 could generate 16.9%higher oleic acid yield than NC-e2.In other words,the oleic acid yield of NC-e2 reached 1.358 times of that of C.gNCF.In conclusion,fadD 5 and fasB proved to be key genes in the fatty acid production pathway of Corynebacterium glutamicum.Knocking out fadD 5 could increase the accumulation of palmitic acid and oleic acid,and additional knocking out fasB could further improve the production of oleic acid.

关 键 词：谷氨酸棒杆菌 油酸 棕榈酸 fadD5 FASB 

分 类 号：Q812[生物学—生物工程]