离子液体辅助超声提取苦参多酚及其体外降血糖活性研究  

Extraction of polyphenols from Sophorae Flavescentis Radix by ionic liquid-assisted ultrasonic method and its hypoglycemic activity in vitro

作　　者：王贞香 李万年 张进举 魏心怀 罗旭 安琼 WANG Zhen-xiang;LI Wan-nian;ZHANG Jin-ju;WEI Xin-huai;LUO Xu;AN Qiong(Medical College of Hexi University;Key Laboratory of Utilisation of Characteristic Resources in Hexi Corridor of Gansu Province,Zhangye 734000,China)

机构地区：[1]河西学院医学院 [2]甘肃省河西走廊特色资源利用重点实验室,张掖734000

出　　处：《天然产物研究与开发》2025年第4期636-646,611,共12页Natural Product Research and Development

基　　金：甘肃省高等学校创新基金(2021A-120);2024年河西学院校长基金(QN2024029);甘肃省大学生省级创新训练项目(S202310740031);国家自然科学基金(22174072)。

摘　　要：探究苦参多酚(polyphenols from Sophorae Flavescentis Radix,SFP)降血糖及抗糖基化活性,以SFP为原料,采用离子液体辅助超声波法提取SFP,通过单因素试验探究离子液体浓度、提取时间、温度、超声功率对SFP提取收率的影响,结合响应曲面设计优化提取条件;在此基础上以泄露曲线、上样浓度、洗脱液最佳浓度、洗脱液体积为考察因素确定AB-8大孔吸附树脂纯化SFP的最佳吸附与解吸条件。并评价SFP对α-葡萄糖苷酶、α-淀粉酶、BSA-葡萄糖模拟体系、BSA-丙酮醛模拟体系的活性。结果表明,在咪唑型离子液体浓度34.6%,料液比1∶35.4(g/mL),提取温度60.6℃,提取时间29.7 min时,SFP平均提取收率可达41.27 mg/g;经AB-8型大孔树脂纯化后,SFP纯度从7.54%提高到了17.47%;并能抑制α-葡萄糖苷酶、α-淀粉酶活性及糖基化反应进而降低血糖。说明离子液体辅助超声提取的SFP经AB-8大孔吸附树脂纯化有较好的体外降糖及抗糖基化能力。This study aims to improve extraction of polyphenols from Sophorae Flavescentis Radix(SFP),which had good ability to lower blood sugar and resist glycation in vitro.The SFP were extracted by ionic liquid-assisted ultrasonic method.The effects of Ionic liquid concentration,extraction time,extraction temperature and ultrasonic power on the extraction yield of SFP were studied by single factor experiment,and the extraction process was optimized by response suriace method based on the resulted of single factor experiment.Meanwhile,α-glucosidase andα-amylase inhibitory activity,and hypoglycemic activity in vitro of SFP was determined.The results showed that the optimal extraction process of SFP was as follows:ionic liquid concentration was 34.6%,material-liquid ratio was 1∶35.4(g/mL),extraction temperature was 60.6℃,extraction time was 29.7 min.The average extraction rate of SFP was 41.27 mg/g by the optimal extraction process,and purified by an AB-8 macroporous resin column,and the purity of SFP was increased from 7.54%to 17.47%.SFP could inhibit the activities ofα-glucosidase andα-amylase,and inhibit the glycosylation reaction to reduce blood glucose,respectively,the SFP extracted by ionic liquid-assisted ultrasonic extraction was purified by AB-8 macroporous adsorption resin,which had good ability to lower blood sugar and resist glycation in vitro.

关 键 词：苦参多酚 提取分离 大孔吸附树脂 体外降血糖 

分 类 号：R284.2[医药卫生—中药学]