木荷化学成分及其抗白色念珠菌活性研究  

Chemical compounds from Schima superba and their anti-Candida albicans activity

作　　者：胡辰 吴春 周光雄 HU Chen;WU Chun;ZHOU Guang-xiong(Pharmacy Department of Gannan Health Vocational College,Ganzhou 341000,China;Key Laboratory of Functional and Clinical Translational Medicine,Fujian Province University,Xiamen Medical College,Xiamen 361023,China;Medicine and Health Science College,Guangzhou Huashang College,Guangzhou 511436,China;Institute of Traditional Chinese Medicine and Natural Products,College of Pharmacy,Jinan University,Guangzhou 510632,China)

机构地区：[1]赣南卫生健康职业学院药学院,赣州341000 [2]厦门医学院机能与临床转化福建省高校重点实验室,厦门361023 [3]广州华商学院健康医学院,广州511436 [4]暨南大学药学院中药和天然药物研究所,广州510632

出　　处：《天然产物研究与开发》2025年第4期659-666,共8页Natural Product Research and Development

基　　金：福建省自然科学基金(2022J05326)。

摘　　要：研究木荷根茎的抗真菌活性成分。采用多种柱色谱技术对木荷根茎乙酸乙酯萃取部位进行了分离纯化,通过NMR、MS等方法鉴定化合物结构,并采用纸片药敏实验法及目测比浊法对分离得到的化合物进行体外抗白色念珠菌活性测试。最终从该木荷提取物的乙酸乙酯萃取部位中共分离得到了8个三萜皂苷类化合物,分别为berneuxia saponin A(1)、sasanquasaponin III(2)、maetenoside B(3)、3-O-{α-L-rhamno-pyranosyl-(1→2)-β-D-galactopyranosyl-(1→3)-[β-D-glucopyranosyl-(1→2)]-β-D-glucuronopyranosyl}primulagenin A(4)、ternstroemiaside A(5)、desacyl-boninsaponin A(6)、sanchakasaponin F(7)、yuchasaponin A(8),以上化合物均为首次从木荷中分离得到。活性结果显示,化合物2、3、7、8显示出不同程度的体外抗白色念珠菌活性。其中,化合物7的抗白色念珠菌活性尤为显著,对白色念珠菌敏感菌株及耐药菌株的最低抑菌浓度值均为0.016 mg/mL,对敏感菌株及耐药菌株的最小杀菌浓度分别为0.132和0.016 mg/mL。In order to investigate the antifungal active components in root and stem of Schima s uperba,compounds were isolated and purified from the ethyl acetate extract using various column chromatography techniques,and their chemical structures were identified with NMR and MS spectroscopic methods.The activity of the isolated compounds against Candida albicans was evaluated in vitro by disk susceptibility assay and visual turbidimetry.Eight triterpenoid saponins were isolated from the EtOAc fraction of the alcoholic extract of S.s uperba,including berneuxia saponin A(1),sasanquasaponin III(2),maetenoside B(3),3-O-{α-L-rhamno-pyranosyl-(1→2)-β-D-galactopyranosyl-(1→3)-[β-D-glucopyranosyl-(1→2)]-β-D-glucuronopyranosyl}primulagenin A(4),ternstroemiaside A(5),desacyl-boninsaponin A(6),sanchakasaponin F(7),yuchasaponin A(8).All compounds were isolated from S.s uperba for the first time.The results indicated that compounds 2,3,7 and 8 showed different degrees of anti-C.albicans activity in vitro.Among them,compound 7 showed notable activity against C.albicans.The minimum inhibitory concentrations against sensitive and drug-resistant strains of C.albicans were 0.016 mg/mL.The minimum fungicidal concentrations of sensitive and resistant strains were 0.132 and 0.016 mg/mL,respectively.

关 键 词：木荷 化学成分 三萜皂苷 抗白色念珠菌活性 

分 类 号：R284.1[医药卫生—中药学]