赛里木湖来源链霉菌Streptomyces sp.A5的次级代谢产物研究  

Secondary metabolites of Streptomyces sp.A5 derived from Sayram Lake

作　　者：周彪 李英 陈晴 余海谦 李超 卞华[1] ZHOU Biao;LI Ying;CHEN Qing;YU Hai-qian;LI Chao;BIAN Hua(Henan Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation,Nanyang Institute of Technology,Nanyang 473004,China;Lei Yunshang Pharmaceutical Group Co.,Ltd.,Suzhou 215009,China)

机构地区：[1]南阳理工学院河南省张仲景方药与免疫调节重点实验室,南阳473004 [2]雷允上药业集团有限公司,苏州215009

出　　处：《天然产物研究与开发》2025年第4期674-677,666,共5页Natural Product Research and Development

基　　金：中原科技创新领军人才项目(234200510006);河南省自然科学基金(222300420248);河南省高等学校重点科研项目(22A360016);南阳理工学院博士科研启动基金项目(NGBJ-2019-25)。

摘　　要：研究赛里木湖来源放线菌Streptomyces sp.A5的次级代谢产物。采用正相硅胶柱、Sephadex LH-20凝胶柱以及半制备高效液相色谱等分离方法,从该菌株的大米固体培养基发酵产物中分离得到5个化合物,通过NMR、MS等波谱方法鉴定为(±)-4′-methoxydehydroaltenusin(1)、alternariol 4-methyl ether(2)、6,8-dihydroxy-3-methylisocoumarin(3)、吲哚-3-甲酸甲酯(4)、streptcytosine H(5)。其中,化合物1为新的脱氢阿霉素类化合物,并对分离得到的化合物进行抗菌活性筛选。结果表明,化合物1对大肠杆菌、金黄色葡萄球菌以及白色念珠菌均呈现出中等的抑制活性,最小抑菌浓度(minimal inhibitory concentration,MIC)分别为12.5、6.25和6.25μg/mL,化合物2对这3种细菌表现出较弱抑制活性,其MIC均为25μg/mL。This study aims to investigate the secondary metabolites of Streptomyces sp.A5 derived from Sayram Lake.Five compounds were isolated from the solid fermentation extract of Streptomyces sp.A5 by using various chromatography methods including silica gel column,Sephadex LH-20 column and semipreparative HPLC.Their structures were determined by NMR,MS methods as a new dehydroaltenusin derivative,namely(±)-4′-methoxydehydroaltenusin(1),and four known compounds,including alternariol 4-methyl ether(2),6,8-dihydroxy-3-methylisocoumarin(3),indole-3-methyl formate(4),streptcytosine H(5).The antibacterial activities of compounds 1-5 were evaluated.The results indicated that compound 1 exhibited moderate antibacterial activity against Escherichia coli,Staphylococcus aureus and Candida albicans with minimal inhibitory concentration(MIC)at 12.5,6.25 and 6.25μg/mL,respectively.Compound 2 showed a weaker antibacterial activity against them with same MIC at 25μg/mL.

关 键 词：赛里木湖 链霉菌A5 化学成分 抗菌活性 

分 类 号：R284.2[医药卫生—中药学]