基于HPLC指纹图谱及UPLC-MS/MS多指标成分含量测定的广西不同产地五指毛桃质量评价  

Quality evaluation of Fici Hirtae Radix from different regions in Guangxi based on HPLC fingerprint and UPLC-MS/MS multi-index component content determination

作　　者：黄春燕 何敏 何秋云 柳贤福[2] 莫元瑶 周怡 莫永福 陈汝旭 HUANG Chun-yan;HE Min;HE Qiu-yun;LIU Xian-fu;MO Yuan-yao;ZHOU Yi;MO Yong-fu;CHEN Ru-xu(Qinzhou Inspection and Testing Centre,Qinzhou 535000,China;Faculty of Chinese Medicine Science Guangxi University of Chinese Medicine,Nanning 530222,China)

机构地区：[1]钦州市检验检测中心,钦州535000 [2]广西中医药大学赛恩斯新医药学院,南宁530222

出　　处：《天然产物研究与开发》2025年第4期678-686,共9页Natural Product Research and Development

基　　金：广西壮族自治区市场监管局科技项目(GSJKJZC2022-19);广西中医药大学赛恩斯新医药学院校级科研项目(2023MS007)。

摘　　要：建立五指毛桃多指标成分的质量控制方法。采用HPLC法建立五指毛桃的指纹图谱,采用“中药色谱指纹图谱相似度评价系统(2012A版)”评价相似度,采用SPSS 22.0对样品进行聚类分析(cluster analysis,CA)和主成分分析(principal component analysis,PCA);采用UPLC-MS/MS测定多指标成分含量。HPLC指纹图谱共标定了24个共有峰,指认其中7个共有峰分别为补骨脂素、槲皮素、柚皮素、木犀草素、佛手柑内酯、芹菜素、金合欢素,相似度为0.990~0.999,分别聚为3类;UPLC-MS/MS分析表明五指毛桃含有8个成分,分别为补骨脂素、槲皮素、柚皮素、木犀草素、佛手柑内酯、山柰素、芹菜素、金合欢素,8个成分各自的质量浓度范围内线性关系良好,平均加样回收率为96.88%~101.0%,RSD为1.2%~1.8%。CA结果显示12批五指毛桃聚为3类。PCA结果显示结果第一主成分(补骨脂素)特征值为11.89,方差贡献率为99.08%,表明该主成分可作为五指毛桃的质量标志物。12批样品8个成分含量分别为0.0858~1.11、2.51×10^(-6)~5.34×10^(-4)、1.24×10^(-3)~0.117、2.30×10^(-4)~3.98×10^(-2)、0.011~0.507、3.36×10^(-6)~8.17×10^(-4)、2.52×10^(-3)~7.92×10^(-2)、5.13×10^(-5)~5.85×10^(-4)mg/g,12批样品各成分含量差异存在一定的差异。综上,研究建立的方法准确度良好,稳定可靠,可为五指毛桃药材的质量控制提供科学的参考依据。This study aims to establish a quality control method for multi-index components of Fici Hirtae Radix.The HPLC fingerprinting was established and its simility was evaluated by“Similarity Evaluation System for Chromatographic Fingerprinting in Chinese Medicine(2012A version)”,and the samples were analysed by SPSS 22.0 for the clustering analysis(CA)and the principal component analysis(PCA),and the content of multi-index components was determined by UPLC-MS/MS.The HPLC fingerprint identified 24 common peaks,of which seven perks were identified as psoralen,quercetin,naringenin,luteolin,bergapten,apigenin,and aconitine.The similarities were 0.990-0.999,and the samples were divided into three categories.The experimental results showed that Fici Hirtae Radix contained eight components by UPLC-MS/MS,including psoralen,quercetin,naringenin,luteolin,bergapten,kaempferol,apigenin,and aconitine.The eight components exhibited excellent linear relationships within their respective mass concentration ranges,the average recoveries were 96.88%-101.0%,and the RSD were 1.2%-1.8%.The CA results showed that the 12 batches of samples were clustered into three groups,the PCA results showed that the eigenvalue of the first principal component(psoralen)was 11.89,and the variance contribution rate was 99.08%,which indicated that this principal component could be regarded as a quality marker for the Fici Hirtae Radix.The content of eight components in 12 batches of samples were 0.0858-1.11,2.51×10^(-6)-5.34×10^(-4),1.24×10^(-3)-0.117,2.30×10^(-4)-3.98×10^(-2),0.011-0.507,3.36×10^(-6)-8.17×10^(-4),2.52×10^(-3)-7.92×10^(-2),5.13×10^(-5)-5.85×10^(-4)mg/g.There were certain differences in the content of each component in the 12 batches of samples.In conclusion,the established method have good accuracy,stability,and reliability,and it can provide a scientific reference for the quality control of Fici Hirtae Radix.

关 键 词：五指毛桃 HPLC UPLC-MS/MS 指纹图谱 多指标成分含量测定 

分 类 号：R917[医药卫生—药物分析学]