层次分析-熵权法结合中心复合设计-响应面法优选高乌头根酒蒸工艺研究  

Study on the optimization of Huangjiu-steamed process of Aconitum sinomontanum Nakai root by CCD-response surface and AHP-entropy weight method

作　　者：马佳荣 王玉辉 李莉[1,2] 赵敏 李芸[1,2,3] MA Jia-rong;WANG Yu-hui;LI Li;ZHAO Min;LI Yun(Gansu University of Chinese Medicine;Gansu Pharmaceutical Industry Innovation Research Institute;Northwest Collaborative Innovation Center for Traditional Chinese Medicine,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学 [2]陇药产业创新研究院 [3]西北中藏药省部共建协同创新中心,兰州730000

出　　处：《天然产物研究与开发》2025年第4期723-731,共9页Natural Product Research and Development

基　　金：国家自然科学基金(82360773)。

摘　　要：探究酒蒸高乌头根的最佳炮制工艺。本研究以总生物碱、高乌甲素及冉乌头碱含量的综合评分作为评价指标,采用紫外分光光度法测定总生物碱含量,超高效液相色谱法测定高乌甲素和冉乌头碱含量。为探究加黄酒量、闷润时间、蒸制时间对酒蒸高乌头根炮制工艺的影响,开展了单因素考察试验。采用层次分析-熵权法确定总生物碱、高乌甲素、冉乌头碱含量的权重,以三者的综合评分结合中心复合设计-响应面法确定酒蒸高乌头根的最佳炮制工艺。结果表明酒蒸高乌头根的最佳炮制工艺为:高乌头根重量15%的加酒量、闷润时间2 h、蒸制时间5 h、蒸制温度127℃、蒸制压力0.15 MPa。本研究拓宽了高乌头根炮制方法且所建立的炮制工艺评价体系准确、可信,为高乌头根炮制相关研究及临床安全提供参考。This study aims to explore the optimal processing parameters of the Aconitum sinomontanum root.The comprehensive score of total alkaloid,lappaconitine and ranaconitine content were used as the evaluation indexes.The total alkaloid content was determined by ultraviolet spectrophotometry,and the lappaconitine and ranaconitine contents were measured by ultra-high performance liquid chromatography.The single factor experiment were conducted to determine the effects of the amount of added Huangjiu,moistening time,and steaming time.The weight of the three components were determined by analytic hierarchy process combined with entropy weight method.The optimal processing parameters of Huangjiu-steamed A.sinomontanum root were determined by the comprehensive score combined with central composite design-response surface method.The results showed that the optimal processing parameters for Huangjiu-steamed A.sinomontanum root was as follows:15%of the weight of A.sinomontanum root as the amount of added Huangjiu,a moistening time of 2 h,a steaming time of 5 h,a steaming temperature of 127℃,and a steaming pressure of 0.15 MPa.This study broadens the processing methods of A.sinomontanum root.The established processing evaluation system for the processing technology is accurate and reliable,and can provide references for related research and clinical safety applications of A.sinomontanum root.

关 键 词：层次分析-熵权法 中心复合设计-响应面法 酒蒸高乌头根 总生物碱 高乌甲素 冉乌头碱 

分 类 号：R283[医药卫生—中药学]