细辛趁鲜切制工艺优化  

Optimization of fresh-cut processing of Asari Radix et Rhizoma

作　　者：何建暄 王浩 于子奇 王英平[1,2] 张瑞 HE Jian-xuan;WANG Hao;YU Zi-qi;WANG Ying-ping;ZHANG Rui(College of Herbal Medicine,Jilin Agricultural University;Changchun Observation and Experiment Station of Crop Germplasm Resources,Changchun 132118,China)

机构地区：[1]吉林农业大学中药材学院 [2]国家作物种质资源长春观测实验站,长春132118

出　　处：《天然产物研究与开发》2025年第4期732-742,共11页Natural Product Research and Development

基　　金：国家重点研发计划(2019YFC1710704)。

摘　　要：优化细辛趁鲜切制工艺,提高细辛的加工效率和质量。以六年生北细辛为原材料,通过单因素试验考察不同干燥方式、切制长度、烘干温度、干燥时间下细辛挥发油、细辛脂素和醇溶性浸出物的含量,确定后期工艺优化参数的考察范围。设计正交试验结合熵权法确定细辛趁鲜切制最优工艺,评价细辛趁鲜切制饮片的镇痛抗炎活性。研究得出细辛趁鲜切制后最优的工艺条件为干燥方式为鼓风干燥,切制长度为2.0 cm,烘干温度为40℃,干燥时间为3 h。细辛趁鲜切制饮片水提物能显著提高小鼠的痛阈值,抑制小鼠扭体次数,抑制率为43.64%。经25、50、100μg/mL的细辛趁鲜切制饮片水提物作用脂多糖诱导的小鼠单核巨噬细胞RAW 264.7,能够显著降低NO分泌量,显著抑制诱导型一氧化氮合酶(inducible nitric oxide sythase,iNOS)和前列腺素内过氧化物合酶2(prostaglandin-endoperoxide synthase 2,PTGS2)的蛋白表达量。实验结果表明细辛趁鲜切制饮片有明显的镇痛抗炎作用,能够更好地保存细辛的药效和质量,提高加工效率。趁鲜切制加工工艺在细辛饮片生产上具有可行性,为细辛产地加工和饮片炮制一体化研究与应用提供参考。This study aims to improve the processing efficiency and quality of Asari Radix et Rhizoma,optimize the fresh-cut technology of Asari Radix et Rhizoma.The single factor test was used to evaluate the amounts of volatile oil,asarinin,and alcohol-soluble extract under various drying methods,cutting length,drying temperature,and drying time using six-year-old Asari Radix et Rhizoma as raw material.An orthogonal experiment and the entropy weight approach were used to determine the most suitable process for fresh-cut processing of Asari Radix et Rhizoma and to assess the analgesic and anti-inflammatory effects of fresh-cut Asari Radix et Rhizoma pieces.The study concluded that the most suitable conditions for fresh-cut processing of Asari Radix et Rhizoma were blast drying,cutting length of 2.0 cm,drying temperature of 40℃,drying time of 3 h.Fresh-cut aqueous extracts of Asari Radix et Rhizoma can significantly increase the pain threshold of mice and inhibited the number of twitches in mice with 43.64%inhibition rate.Fresh-cut aqueous extracts of Asari Radix et Rhizoma at 25μg/mL,50μg/mL,and 100μg/mL inhibited NO secretion,inducible nitric oxide sythase and prostaglandin-endoperoxide synthase 2 protein expression in lipopolysaccharide-induced mice monocyte macrophage RAW 264.7 cells.The experimental results showed that the fresh-cut Asari Radix et Rhizoma pieces have obvious analgesic and anti-inflammatory effects,which can better preserve the efficacy and quality of Asari Radix et Rhizoma and improve the processing efficiency.It provides reference for the research and application of the integration of Asari Radix et Rhizoma processing and decoction piece processing.It is feasible to use the fresh-cut processing in the production of Asari Radix et Rhizoma,which can provide a reference for the research and application of the integration of Asari Radix et Rhizoma processing pieces and tablet concocting.

关 键 词：细辛 趁鲜切制 熵权法 脂多糖 

分 类 号：R283[医药卫生—中药学]