基于谱效关系和网络药理学的苗药血人参抗炎活性成分研究  

Study on the anti-inflammatory active substances of Miao medicine Indigofera stachyodes Lindl.based on spectrum-effect relationship and network pharmacology

作　　者：聂琴 吴静澜 王丹 张均航 娄华勇 徐剑 张永萍 傅建 NIE Qin;WU Jing-lan;WANG Dan;ZHANG Jun-hang;LOU Hua-yong;XU Jian;ZHANG Yong-ping;FU Jian(School of Pharmacy,Guizhou University of Traditional Chinese Medicine;National Miao Medicine Engineering Technology Research Centre;Guizhou Traditional Chinese Medicine Processing and Preparation Engineering Technology Research Centre,Guiyang 550025,China;Natural Products Research of Guizhou Province,Guiyang 550014,China)

机构地区：[1]贵州中医药大学药学院 [2]国家苗药工程技术研究中心 [3]贵州中药炮制与制剂工程技术研究中心,贵阳550025 [4]贵州省天然产物研究中心,贵阳550014

出　　处：《天然产物研究与开发》2025年第4期756-767,731,共13页Natural Product Research and Development

基　　金：贵州省科技计划(黔科合支撑[2021]一般411);国家苗药工程技术研究中心能力提升项目(黔科合中引地[2023]006);贵州省高层次创新型人才项目(千层次及黔科合平台人才-GCC[2023]037)。

摘　　要：为明确苗药血人参的化学成分及抗炎活性物质,本研究通过建立血人参UPLC指纹图谱方法,利用化学计量法对其成分进行比较分析,结合网络药理学-分子对接预测血人参抗炎的活性有效靶点,并采取体外细胞实验对其抗炎药效进行验证。血人参指纹图谱结合化学计量法分析结果显示,不同产地血人参共得到28个共有峰,其中指认了10个共有峰,并首次指认出血人参所含成分牡荆素、木犀草苷、金丝桃苷和汉黄芩素。聚类分析可将不同产地血人参聚为3类,主成分分析与聚类分析结果一致,可相互佐证。体外细胞实验表明,不同产地的血人参对炎症细胞有不同程度的修复作用。网络药理学结果表明,表皮生长因子受体(epidermal growth factor receptor,EGFR)和过氧化物酶体增殖物激活受体(peroxisome proliferator-activated receptor gamma,PPARG)是抗炎潜在的靶点,EGFR酪氨酸激酶抑制剂耐药性被认为是抗炎的主要途径之一。本研究建立了血人参的谱-效关系,并结合网络药理学阐明血人参抗炎的关键靶点及作用通路,为血人参的质量控制和品质评价提供参考依据。To elucidate the chemical compositions and anti-inflammatory active substances of Miao medicine Indigofera stachyodes(I.stachyodes),an UPLC fingerprint method was established.Stoichiometry method was employed to analyze its components,then the effective anti-inflammatory targets of I.stachyodes was predicted via network pharmacology combined with molecular docking and its anti-inflammatory efficacy was further verified through in vitro cell experiments.The fingerprint spectrum of I.stachyodes integrated with chemical stoichiometry analysis displayed that a total of 28 common peaks were obtained,of which ten common peaks were identified.Vitexin,luteolin,hyperoside and baicalein were identified from I.stachyodes for the first time.Cluster analysis exhibited that I.stachyodes from different origins can be divided into three categories,and the results of principal component analysis is consistent with cluster analysis.Furthermore,in vitro cell assay exhibited that I.stachyodes from different origins with varying degrees of reparative effects.Network pharmacology studies demonstrated that the epidermal growth factor receptor(EGFR)and peroxisome proliferator-activated receptor gamma(PPARG)were potential targets,and EGFR tyrosine kinase inhibitor resistance was considered one of the primary pathways for anti-inflammatory.Collectively,this study successfully developed the spectrum-effect relationship of I.stachyodes,in addition,the signaling pathways and key targets of I.stachyodes anti-inflammatory were elucidated in conjunction with network pharmacology.These results will provide a substantial basis for quality control and evaluation of I.stachyodes.

关 键 词：苗药血人参 超高效液相色谱 谱效关联 网络药理学 分子对接 

分 类 号：R932[医药卫生—生药学]