桑白皮多糖提取工艺的研究  

Optimization of extraction process for Cortex Mori polysaccharide

作　　者：于淼 肖念桐 蒋海生 李春宵 董翠翠 任振堃 史东辉 王佳丽 Yu Miao;Xiao Niantong;Jiang Haisheng;Li Chunxiao;Dong Cuicui;Ren Zhenkun;Shi Donghui;Wang Jiali(College of Animal Science and Veterinary Medicine,Jinzhou Medical University,Liaoning Jinzhou 121001;Animal Disease Prevention and Control Center of Anshan City,Liaoning Anshan 114000;Xiuyan Manchu Autonomous County Agricultural Comprehensive Law Enforcement Brigade,Liaoning Anshan 114300;The Third Affiliated Hospital of Jinzhou Medical University,Liaoning Jinzhou 121001)

机构地区：[1]锦州医科大学畜牧兽医学院,辽宁锦州121001 [2]鞍山市动物疫病预防控制中心,辽宁鞍山114000 [3]岫岩满族自治县农业综合行政执法大队,辽宁鞍山114300 [4]锦州医科大学附属第三医院,辽宁锦州121001

出　　处：《现代畜牧兽医》2025年第3期7-10,共4页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：辽宁省教育厅基本科研项目(LJKFZ20220246);辽宁省科技攻关项目(2021JH1/10400033);锦州医科大学2024年大学生创新训练项目(202410160006、X202410160075);锦州医科大学2024年大学生创业实践项目(X202410160005S)。

摘　　要：试验旨在优化桑白皮多糖(Cortex Mori polysaccharide,CMP)的提取工艺,为畜禽生产中合理利用CMP作为免疫增强剂提供参考。试验通过水提醇沉工艺提取CMP,在单因素试验的基础上,以CMP得率为评价指标设计正交试验,优选CMP提取条件。结果显示,影响CMP得率因素的主次顺序为提取温度>料液比>提取时间;CMP的最佳提取条件为提取温度90℃、液料比20 mL/g、提取时间3 h,此条件下测得桑白皮粗提物中多糖含量为59.29%,CMP得率为6.65%。研究表明,试验建立的工艺具有较高的提取效率,为CMP的进一步研究奠定了一定基础。The experiment aimed to optimize the extraction technology of Cortex Mori polysaccharide(CMP)to provide a reference for the rational use of CMP as an immune enhancer in livestock and poultry production.CMP was extracted via the water extraction and alcohol precipitation method.Building on preliminary single-factor experiments,an orthogonal test was designed with CMP yield as the evaluation criterion to optimize extraction parameters.Results demonstrated that extraction temperature exerted the most significant influence on CMP yield,followed by liquid-to-material ratio and extraction time.The optimal extraction conditions were determined as follows:a temperature of 90℃,a liquid-to-material ratio of 20 mL/g,and an extraction duration of 3 hours.Under these optimized parameters,the polysaccharide content in the crude extract reached 59.29%,with a CMP yield of 6.65%.This systematic approach highlights the critical role of temperature control in enhancing extraction efficiency while providing actionable insights for scaling up CMP production processes.The results show that the process has higher extraction efficiency,which lays a foundation for the further study of CMP.

关 键 词：桑白皮 多糖 提取工艺 

分 类 号：S816.7[农业科学—饲料科学]