1株鸽源唾液乳杆菌的分离鉴定及生物学特性分析  

Isolation,identification,and biological characteristics analysis of a pigeon-derived Lactobacillus salivarius strain

作　　者：党乙凡 牛梅婷 李涛[1] 张蒙林 陈荣[1,2,3] Dang Yifan;Niu Meiting;Li Tao;Zhang Menglin;Chen Rong(College of Animal Science and Technology,Tarim University,Xinjiang Alar 843300;Key Laboratory of Circum-Tarim Livestock and Grass Resources Utilization of the Ministry of Agriculture and Rural Affairs,Xinjiang Alar 843300;Engineering Laboratory for Diagnosis and Control of Animal Diseases in Tarim,Xinjiang Production and Construction Corps,Xinjiang Alar 843300)

机构地区：[1]塔里木大学动物科学与技术学院,新疆阿拉尔843300 [2]农业农村部环塔里木畜草资源利用重点实验室,新疆阿拉尔843300 [3]新疆生产建设兵团塔里木动物疫病诊断与防控工程实验室,新疆阿拉尔843300

出　　处：《现代畜牧兽医》2025年第3期21-25,共5页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：兵团指导性科技计划项目(2022ZD111、TDZKKY-202203)。

摘　　要：试验旨在筛选专用于肉鸽的乳酸菌菌株,对采集自鸽舍肉鸽的口腔咽拭子进行病原体的分离鉴定、形态学观察、生化鉴定和16S rDNA序列分析,并对分离株进行耐酸、耐胆盐、耐温以及抑菌性能研究。结果显示,分离株经形态学观察、生化鉴定及16S rDNA序列分析鉴定为唾液乳杆菌并命名为C-1-3-1。分离株C-1-3-1在40、45℃条件下存活率均达到70%以上,可在含0.20%猪胆盐的MRS培养基中少量存活;培养基pH值为4时,菌株存活率达到63.0%,pH值为5时,菌株的存活率超过90%。分离株可抑制大肠杆菌、金黄色葡萄球菌生长,且与败酱草提取液的联合抑菌效果更佳。研究表明,分离株C-1-3-1具有不错的耐温、耐酸、耐胆盐能力以及良好的益生菌特性,可作为益生菌的候选菌株。The experiment aimed to screen Lactobacillus strains specifically for meat pigeons.Pharyngeal swabs collected from meat pigeons in the poultry house were subjected to pathogen isolation and identification,morphological observation,biochemical characterization,and 16S rDNA sequence analysis.Additionally,the isolated strains were studied for acid tolerance,bile salt tolerance,thermal tolerance,and antimicrobial activity.The results showed that the isolate was identified as Lactobacillus salivarius through morphological observation,biochemical characterization,and 16S rDNA sequence analysis,and was designated as C-1-3-1.The survival rate of isolate C-1-3-1 exceeded 70%at 40℃and 45℃,and it exhibited limited survival in MRS medium containing 0.20%porcine bile salt.At pH 4,the strain's survival rate reached 63.0%,while at pH 5,the survival rate exceeded 90%.The isolate inhibited the growth of Escherichia coli and Staphylococcus aureus,with enhanced antimicrobial effects when combined with Patrinia scabiosifolia extract.The study demonstrated that isolate C-1-3-1 possesses strong thermal tolerance,acid tolerance,bile salt tolerance,and promising probiotic properties,making it a potential candidate for probiotic development.

关 键 词：唾液乳杆菌 分离鉴定 败酱草提取液 抑菌性能 

分 类 号：S816[农业科学—饲料科学]