长春市汉族人群维生素D受体基因多态性、TGF-β、TRACP与腰椎骨密度的相关性  

Study on the correlation between vitamin D receptor gene polymorphism,TGF-β,TRACP and lumbar bone density in the Han population of Changchun city

作　　者：马倩倩 毛未贤 高远 尹纪伟 程海涛 MA Qianqian;MAO Weixian;GAO Yuan;YIN Jiwei;CHENG Haitao(Jilin FAW General Hospital,Changchun 130011,China)

机构地区：[1]吉林省一汽总医院,吉林长春130011

出　　处：《中国骨质疏松杂志》2025年第4期490-494,共5页Chinese Journal of Osteoporosis

基　　金：吉林省卫生健康技术创新项目(2019J092)。

摘　　要：目的研究长春市35~79岁汉族人群维生素D受体基因BsmⅠ、FokⅠ位点多态性的分布特征及其与腰椎骨密度(bone mineral density,BMD)的相关性,分析不同基因型对骨代谢的调节与影响,同时研究血清TGF-β、TRACP水平与腰椎BMD的相关性,为骨质疏松预防、早期诊断和治疗提供分子生物学依据。方法应用Hologic Discovery WA型骨密度仪检测腰椎正位(L1-L4)BMD;采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析BsmⅠ和FokⅠ位点多态性;采用酶联免疫吸附分析法检测血清TGF-β、TRACP水平;采用SPSS 23.0软件进行统计分析。结果在500名汉族人群中,BsmⅠ位点基因型以bb型为主,占80.2%,Bb型占15.2%,BB型占4.6%;FokⅠ位点ff基因型占18.6%,Ff型占45.8%,FF型占35.6%。BsmⅠ位点各基因型骨质疏松组与非骨质疏松组分布特征组间差异无统计学意义(P>0.05);FokⅠ位点ff基因型骨质疏松组所占比例高于非骨质疏松组,差异有统计学意义(P<0.05)。BsmⅠ位点bb基因型男性、女性BMD值均低于Bb型和BB型,但差异无统计学意义(P>0.05);FokⅠ位点ff基因型男性、女性BMD值均低于Ff型和FF型,差异有统计学意义(P<0.05)。血清TGF-β水平骨质疏松组显著低于非骨质疏松组,而TRACP水平骨质疏松组高于非骨质疏松组,组间差异有统计学意义(P<0.05)。结论本研究500名汉族人群中,BsmⅠ位点以bb基因型为主,占80.2%,各基因型骨密度值组间差异无统计学意义;FokⅠ位点ff型BMD值低于Ff型和FF型,骨质疏松组ff基因型所占比例高于非骨质疏松组,差异有统计学意义,提示ff基因型可能是骨质疏松发生的危险因素;骨质疏松组血清TGF-β水平显著低于非骨质疏松组,而TRACP水平高于非骨质疏松组,差异有统计学意义,表明TGF-β、TRACP是评价骨代谢状态的良好指标。Objective Study the distribution characteristics of vitamin D receptor gene BsmⅠand FokⅠpolymorphism in the Han population aged 35-79 in Changchun city and their correlation with lumbar bone mineral density(BMD),analyzing the regulation and influence of different genotypes on bone metabolism.Simultaneously investigate the correlation between serum TGF-β,TRACP levels and lumbar BMD.In order to provide molecular biology basis for the prevention,early diagnosis and treatment of osteoporosis.Methods Hologic Discovery WA bone densitometer was used to detect lumbar spine anteroposterior(L1-L4)BMD;polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP)was used to analyze the polymorphism of BsmⅠand FokⅠ;Application of enzyme-linked immunosorbent assay to detect serum TGF-βand TRACP levels;Statistical analysis was conducted using SPSS 23.0 software.Results Among the 500 Han population,the genotype of BsmⅠwas mainly bb type,accounting for 80.2%,Bb type accounted for 15.2%,and BB type accounted for 4.6%;ff genotype of FokⅠaccounted for 18.6%,Ff genotype accounted for 45.8%,and FF genotype accounted for 35.6%.There was no statistically significant difference(P>0.05)in the distribution characteristics of each genotype of BsmⅠbetween the osteoporosis group and the non osteoporosis group;The proportion of FokⅠff type in osteoporosis group was higher than that in non osteoporosis group,and the difference is statistically significant(P<0.05).The BMD values of both males and females with the bb genotype of BsmⅠwere lower than those of the Bb and BB genotypes,but there was no statistically significant difference(P>0.05);The BMD values of both males and females with the FokⅠff genotype were lower than those of the Ff and FF genotypes,and the difference was statistically significant(P<0.05).The serum TGF-βlevel in the osteoporosis group was significantly lower than that in the non osteoporosis group,while the TRACP level was higher than that in the non osteoporosis group,and the difference

关 键 词：维生素D 受体基因 BsmⅠ FokⅠ 转化生长因子-β 抗酒石酸酸性磷酸酶 骨密度 

分 类 号：R589[医药卫生—内分泌]