复方羟氯扎胺混悬液对藏羊肝片吸虫和线虫的驱虫效果  

Clinical efficacy evaluation of compound oxyclozanide suspension on liver fluke and nematode disease in Tibetan sheep

作　　者：姚学强 王娟 李江 周凯仁 翟斌涛 张继瑜[1] 周绪正[1] YAO Xueqiang;WANG Juan;LI Jiang;ZHOU Kairen;ZHAI Bintao;ZHANG Jiyu;ZHOU Xuzheng(Lanzhou Institute of Husbandry and Veterinary Pharmaceutical,Chinese Academy of Agricultural Sciences/Key Laboratory of Veterinary Pharmaceutical Development,Ministry of Agriculture and Rural Affairs/Key Laboratory of New Animal Drug Project of Gansu Province,Lanzhou 730050,China)

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所/农业农村部兽用药物创制重点实验室/甘肃省新兽药工程重点实验室,甘肃兰州730050

出　　处：《畜牧与兽医》2025年第5期114-121,共8页Animal Husbandry & Veterinary Medicine

基　　金：国家重点研发计划项目(2022YFD1602201);甘肃省科技计划项目(20JR10RA023)。

摘　　要：旨在通过复方羟氯扎胺混悬液(oxyclozanide-suspension)对藏羊的驱虫试验,评价复方羟氯扎胺混悬液对藏羊肝片吸虫和消化道线虫的临床药效,为确定该复方制剂的临床最佳推荐剂量提供科学依据。筛选出150只患有肝片吸虫病和消化道线虫病的羊,随机分成5组,每组30只,分别为复方羟氯扎胺混悬液(羟氯扎胺+左旋咪唑)低剂量(0.125 mL/kg)、中剂量(0.25 mL/kg)、高剂量(0.5 mL/kg)组,进口药物对照(复方羟氯扎胺混悬液Levafas Diamond 0.25 mL/kg)和空白对照(发病不给药)组。给药前及给药后每周根据试验羊的临床状况、每克粪便的虫卵数(EPG)、虫卵减少率、虫卵转阴率等指标评价复方羟氯扎胺混悬液的治疗效果。结果:试验结束后增重结果显示低剂量组、中剂量组、高剂量组和药物对照组的羊与空白对照组相比差异显著(P<0.05),试验期间各试验组和进口药物对照组的羊体重均随时间延长而增加,空白对照组羊体重略有减少;给药后第14天对肝片吸虫驱虫效果观察发现,低剂量组、中剂量组、高剂量组、药物对照组和空白对照组粪便虫卵减少率依次为99.5%、100%、100%、100%和0,虫卵转阴率依次为96.7%、100%、100%、100%和0;整个试验期间复方羟氯扎胺混悬液对消化道奥斯特线虫、细颈线虫、毛圆线虫和捻转血矛线虫均有明显的驱杀效果,给药后第14天试验结果显示,低剂量组、中剂量组、高剂量组、药物对照组和空白对照组粪便中线虫虫卵减少率依次为99.6%、100%、100%、100%和0,虫卵转阴率依次为93.3%、100%、100%、100%和0。结果表明,复方羟氯扎胺混悬液一次用药对羊肝片吸虫和消化道线虫均有显著的治疗作用,其临床最适推荐剂量为0.25 mL/kg。This study was to evaluate the clinical efficacy of compound oxyclozanide suspension on Fasciola hepatica and gastrointestinal nematodes in Tibetan sheep through anthelmintic test of oxyclozanide-levamisole,so as to provide a scientific basis for determining the optimal recommended clinical dose of the compound preparation.150 sheep with liver fluke disease and gastrointestinal nematode disease were selected and randomly divided into 5 groups,with 30 sheep in each group.The groups were the low dose(0.125 mL/kg),medium dose(0.25 mL/kg),high dose(0.5 mL/kg),imported drug control(oxyclozanide suspension Levafas Diamond 0.25 mL/kg),and blank control groups.The therapeutic effect of compound hydroxyclozamide suspension was evaluated before and weekly after administration based on clinical conditions,EPG values,egg reduction rate and egg seroconversion rate of the experimental sheep.After the experiment,the weight gain results of sheep showed significant differences(P<0.05) between the low dose,medium dose,high-dose,and drug control and blank control groups.During the experiment,the weight of the sheep in each experimental group and the imported drug control group increased with time,while the weight of the sheep in the blank control group slightly decreased.After 14 days of administration,the observation of the deworming effect on liver fluke showed that the reduction rates of the fecal worm eggs in the low,medium,and high dose groups of compound hydroxyclozamide suspension,the drug control group,and the blank control group were 99.5%,100%,100%,100%,and 0,respectively.The egg conversion rates of each group were 96.7%,100%,100%,100%,and 0,respectively.During the entire trial period,the com-pound hydroxycloxamide suspension had a significant repellent effect on gastrointestinal nematodes such as Osterhabditis,filamentous nema-todes,round nematodes,and twisted blood nematodes.On the 14^(th)day of the administration,the test results showed that the reduction ratesof the fecal nematode eggs in the low,medium,and high-dose

关 键 词：藏羊 肝片吸虫 线虫 复方羟氯扎胺混悬液 药效学 

分 类 号：S858[农业科学—临床兽医学]