防治猫腹泻的中药复方片剂的制备及对腹泻的治疗效果  

Preparation and application of traditional Chinese medicine compound tablets for preventing and treating diarrhea in cats

作　　者：孔祥瑞 于亚明 贺瑾 田维鹏 毛宁宁 刘欣 卢炫圻 王德云[1] KONG Xiangrui;YU Yaming;HE Jin;TIAN Weipeng;MAO Ningning;LIU Xin;LU Xuanqi;WANG Deyun(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;Nutrition Research and Development Center of Gambol Pet Group Co.,Ltd.,Liaocheng 252000,China)

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]乖宝宠物食品集团股份有限公司宠物营养研究与发展中心,山东聊城252000

出　　处：《畜牧与兽医》2025年第5期122-131,共10页Animal Husbandry & Veterinary Medicine

摘　　要：为探究中药复方片剂制备方法及对猫腹泻的治疗效果,采用单因素试验挑选片剂所需辅料并确定处方,所制成片通过《2020版兽药典》片剂检测标准,同时加入适口性检测;临床筛选出具有腹泻疾病的宠物猫21只,随机分为低剂量、高剂量和腹泻组,每组7只,另设7只健康猫为健康组,其中腹泻组和健康组猫不予治疗,低剂量组和高剂量组分别给药2片/d和3片/d,使用上述制备的中药复方片剂进行7 d治疗,在第7天收集健康组、腹泻组和高剂量组猫的新鲜粪便用于16S rRNA测序,统计分析猫腹泻的治愈率、治愈后的腹泻分数以及肠道菌群的改变情况。结果显示:中药复方片剂制备所需辅料为微晶纤维素、羧甲基淀粉钠、30%乙醇、硬脂酸镁,矫味剂采用牛肉香精,制备成品为0.45 g/片,其原料药含药量达到63.5%,按《2020版兽药典》要求检测中药复方片剂的重量差异、脆碎度、崩解时间均满足要求;对筛选的21只腹泻猫进行为期7 d的临床治疗,发现腹泻组猫未恢复到健康标准,低剂量组猫在第6天全部恢复,治愈率为85.7%,高剂量组的猫在第4天全部恢复,治愈率为100%;同时使用中药复方片剂组在门水平上拟杆菌门(Bacteroidetes)、梭杆菌门(Fusobacteriota)和放线菌门(Actinobacteriota)增多,厚壁菌门(Firmicutes)减少,在属水平上经黏液真杆菌(Blautia)升高。结论:中药复方片剂能有效防治猫腹泻,调节猫的肠道菌群,增加菌群的丰富度和菌群量,为开发治疗猫腹泻的新药物提供临床依据。Diarrhea is one of the common diseases that occur in cat breeding,which seriously affects the health of cats and leads to a decline in their physical functions.In order to reduce the incidence of diarrhea in cats,this study aimed to investigate methods for preparation of traditional Chinese medicine compound tablets and to determine their therapeutic effects on cats.A single factor experiment was used to select the required excipients for the tablets and to determine their prescription.The resulting tablet passed the tablet testing standards in the 2020 edition of the Veterinary Pharmacopoeia,and palatability testing was also conducted.21 cats with diarrhea disease were clinically screened and randomly divided into the diarrhea group,the low-dose group,and the high-dose group,with 7 cats in each group.At the same time,7 healthy cats were selected as the healthy group.The cats in the diarrhea group and the healthy group were not treated.The low-dose group was treated with 2 tablets per day,and the high-dose group was treated with 3 tablets per day.The traditional Chinese medicine compound tablets prepared above were used for 7 days of treatment.Then,samples of fresh feces from the healthy group,the diarrhea group,the high-dose group of cats were collected on the seventh day for 16S rRNA sequencing.Finally,the cure rate of cat diarrhea,the diarrhea score after cure,and changes in gut microbiota were statistically analyzed.The results showed that the excipients needed for the preparation of the compound tablet of traditional Chinese medicine were microcrystalline cellulose,sodium carboxymethyl starch,30% ethanol and magnesium stearate.The beef essence was used as the flavoring agent,and the finished product was 0.45 g/tablet.The drug content in the source drug substance reached 63.5%.Next,the weight difference,fragility,and disintegration time of the compound tablet of traditional Chinese medi-cine were tested according to the requirements of the Veterinary Pharmacopoeia 2020.21 selected diarrhea cats underwent clin

关 键 词：猫 腹泻 中药片剂 肠道菌群 

分 类 号：S857[农业科学—临床兽医学]