基于D-loop区和cox1基因序列的江西9个地区草鱼养殖群体遗传多样性分析  

An evaluation of genetic diversity parameters in Ctenopharyngodon idellus farming populations from nine Jiangxi regions,based on mitochondrial D-loop and cox1 gene sequences

作　　者：侯佳浩 刘文玉 吴春林 张明辉 李琳洁 王子予 黄培荧 范洪祥 简少卿[1] 赵大显[1] HOU Jiahao;LIU Wenyu;WU Chunlin;ZHANG Minghui;LI Linjie;WANG Ziyu;HUANG Peiying;FAN Hongxiang;JIAN Shaoqing;ZHAO Daxian(Key Laboratory for Aquatic Germplasm Innovation and Utilization of Jiangxi Province,School of Life Sciences,Nanchang University,Nanchang 330031,China;Ji’an City Agricultural and Rural Industry Development Service Center,Ji’an Jiangxi 343000,China)

机构地区：[1]南昌大学生命科学学院水产种质创新与利用江西省重点实验室,江西南昌330031 [2]吉安市农业农村产业发展服务中心,江西吉安343000

出　　处：《南昌大学学报(理科版)》2025年第2期198-207,共10页Journal of Nanchang University(Natural Science)

基　　金：国家重点研发计划“海洋农业与淡水渔业科技创新”重点专项(2023YFD2401800);江西省现代农业产业技术体系(JXARS-15)。

摘　　要：为评估江西省草鱼(Ctenopharyngodon idellus)养殖群体的种质资源现状,本研究采用线粒体D-loop区(309 bp)与细胞色素C氧化酶I(cox1)基因(527 bp)部分序列,对江西省9个草鱼繁育场(新干XG、吉安JA、峡江XJ、大余DY、宁都ND、瑞昌RC、上栗SL、万载WZ、南昌NC)草鱼养殖群体进行遗传多样性分析。结果显示:D-loop区检测到225个多态性位点,分离出27个单倍型;cox1基因检测到73个多态性位点,分离出9个单倍型。两个标记的遗传多样性参数均处于较低水平:D-loop区单倍型多样性(Hd)0.067~0.515,核苷酸多样性(π)0.00012~0.03138,cox1基因Hd=0~0.331,π=0~0.00797。群体结构分析表明,群体间发生了不同程度的分化。遗传分化系数:D-loop区0~0.04783、cox1基因0~0.18190。分子方差分析(AMOVA)表明遗传变异主要源于群体内(D-loop区98.82%,cox1基因97.87%)。单倍型网络图与邻接法系统发育树均未呈现地理聚类特征,表明江西9个地区草鱼养殖群体遗传多样性较低,群体间分化匮乏。本研究中9个地区草鱼养殖群体遗传多样性背景,可以为江西草鱼种质资源保护与利用提供参考依据。To assess the current status of germplasm resources in Ctenopharyngodon idellus farming populations in Jiangxi Province,this study employed partial sequences of the mitochondrial D-loop region(309 bp)and cytochrome c oxidase I(cox1)gene(527 bp)to analyze the genetic diversity of grass carp populations from nine breeding farms in Jiangxi Province(Xingan XG,Jian JA,Xiajiang XJ,Dayu DY,Ningdu ND,Ruichang RC,Shangli SL,Wanzai WZ,Nanchang NC).The results revealed 225 polymorphic sites in the D-loop region,yielding 27 haplotypes,and 73 polymorphic sites in the cox1 gene,yielding nine haplotypes.Genetic diversity parameters for both markers were at low levels:haplotype diversity(Hd)for the D-loop region ranged from 0.067 to 0.515,nucleotide diversity(π)ranged from 0.00012 to 0.03138;for the cox1 gene,Hd ranged from 0 to 0.331,andπranged from 0 to 0.00797.Population structure analysis indicated varying degrees of differentiation among populations.Genetic differentiation coefficients were 0 to 0.04783 for the D-loop region and 0 to 0.18190 for the cox1 gene.Analysis of molecular variance(AMOVA)showed that genetic variation primarily originated within populations(98.82%for the D-loop region and 97.87%for the cox1 gene).Haplotype network diagrams and neighbor-joining phylogenetic trees did not exhibit geographic clustering patterns.These findings suggested that the genetic diversity of Ctenopharyngodon idellus farming populations in the nine regions of Jiangxi is low,with limited differentiation among populations.The genetic diversity background of these populations provides a reference for the conservation and utilization of Ctenopharyngodon idellus germplasm resources in Jiangxi Province.

关 键 词：草鱼 D-LOOP区 cox1基因 遗传多样性 

分 类 号：S917.4[农业科学—水产科学]