牡丹花瓣粉对小鼠急性乙醇性多器官损伤的保护作用  

Protective effect of peony petal powder on acute ethanol-induced multiple organ injury in mice

作　　者：李雨欣 杨旭[1] 谢艳华 王四旺 LI Yuxin;YANG Xu;XIE Yanhua;WANG Siwang(Faculty of Life Sciences and Medicine,Northwest University,Xi'an 710069,China)

机构地区：[1]西北大学生命科学与医学部,陕西西安710069

出　　处：《空军军医大学学报》2025年第4期426-432,共7页Journal of Air Force Medical University

基　　金：陕西省重点研发计划项目(2023-LL-QY-39)。

摘　　要：目的探讨牡丹花瓣冻干极细粉(PFSP)对急性乙醇性损伤的多器官保护作用,为PFSP进一步开发成为解酒和乙醇性多器官损伤保护产品提供理论依据。方法80只小鼠随机分成对照、模型、联苯双酯、PFSP不同剂量等共8组,每组10只。对照组与模型组小鼠灌胃等容量蒸馏水,其余组依次灌胃150 mg/kg联苯双酯和20、40、80、160、320 mg/kg PFSP,1次/d,连续10 d。于末次给药后30 min,除对照组小鼠外,其余各组灌胃为15 mL/kg的560 mL/L乙醇,建立小鼠急性肝损伤模型,8 h后摘眼球取血,取脏器。生化法检测血清中谷草转氨酶(AST)和谷丙转氨酶(ALT)活性,检测肝组织匀浆甘油三酯(TG)、丙二醛(MDA)、超氧化物歧化酶(SOD)、乙醇脱氢酶(ADH)活性;胃组织匀浆中谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性;心组织匀浆中肌酸激酶(CK)、乳酸脱氢酶(LDH)活性;脑组织匀浆中谷胱甘肽(GSH)、一氧化氮(NO)活性;并对小鼠进行了肝、胃、心、脑病理组织学观察。结果PFSP各组小鼠血清中AST、ALT的含量均显著降低并呈现一定剂量效应(P<0.01),即随剂量增加其含量降低更明显;肝和胃系数基本处于正常水平;肝组织中TG、MDA含量明显低于模型组(P<0.05),而ADH活力和SOD含量显著高于模型组(P<0.05);除20 mg/kg的PFSP组外胃组织中GSH-Px含量显著增加且呈现一定剂量效应(P<0.01),胃组织中的CAT含量增加但不具有显著性差异;心组织中CK和LDH活力减小(P<0.05);脑组织中GSH含量升高,NO含量减少但是不具有显著性差异;肝、胃、心、脑等组织病理切片观察证实,PFSP各组与模型组相比,对水肿、炎症的改变显著。结论PFSP具有明显防治乙醇所致小鼠急性肝、胃等损伤的作用。Objective To explore the protective effect of peony petals freeze-dried superfine powder(PFSP)on acute ethanol-induced multiple organ injury,and to provide a theoretical basis for further development of PFSP as a protective product for anti-alcohol and ethanol-induced multiple organ injury.Methods A total of 80 mice were randomly divided into 8 groups,including control group,model group,bifendate group,and PFSP groups with different doses,with 10 mice in each group.Mice in control group and model group were gavaged with equal volume of distilled water,and the remaining groups were gavaged with 150 mg/kg bifendate and 20,40,80,160,and 320 mg/kg PFSP,once a day for 10 d.Except for the control group,the remaining groups were gavaged with 15 mL/kg 560 mL/L ethanol to establish a mouse model of acute liver injury 30 min after the last administration,and blood was taken from the eyeballs and organs after 8 h.The activities of aspartate aminotransferase(AST)and alanine aminotransferase(ALT)in serum were detected by biochemical methods.The activities of triglyceride(TG),malondialdehyde(MDA),superoxide dismutase(SOD)and alcohol dehydrogenase(ADH)in liver tissue homogenates,glutathione peroxidase(GSH-Px),catalase(CAT)in gastric tissue homogenates,creatine kinase(CK),lactate dehydrogenase(LDH)in cardiac tissue homogenates,and glutathione(GSH),nitric oxide(NO)in brain tissue homogenates were detected.The histopathologic observation of liver,stomach,heart and brain was carried out on mice.Results The levels of AST and ALT in the serum of mice in PFSP groups were significantly reduced and showed a certain dose effect(P<0.01),that is,the content decreased more significantly with increasing dose.The liver and stomach coefficients were basically at normal levels.The content of TG and MDA in liver tissue were significantly lower than those in the model group(P<0.05),while the activity of ADH and the content of SOD were significantly higher than those in the model group(P<0.05).Except for the PFSP group of 20 mg/kg,the content of G

关 键 词：牡丹花瓣冻干极细粉 急性乙醇性损伤 急性肝损伤模型 小鼠 

分 类 号：R285.5[医药卫生—中药学]