芩百清肺浓缩丸通过TNF-α/NLRP3/Caspase-1信号通路对感染后咳嗽大鼠气道高反应性的影响  

Qinbai Qingfei Concentrated Pill's Impact on Airway Hyperresponsiveness in Post-infectious Cough Rats via the TNF-α/NLRP3/Caspase-1 Signaling Pathway

作　　者：王彬羽 贾维刚 徐玉龙 刘婕妤 张弛 宋小晗 周迎春[2] Wang Binyu;Jia Weigang;Xu Yulong;Liu Jieyu;Zhang Chi;Song Xiaohan;Zhou Yingchun(Heilongjiang Academy of Traditional Chinese Medicine,Heilongjiang,Harbin 150001,China)

机构地区：[1]黑龙江省中医药科学院,黑龙江哈尔滨150001 [2]黑龙江中医药大学,黑龙江哈尔滨150004

出　　处：《中国中医急症》2025年第4期598-601,615,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：黑龙江省自然科学基金项目(LH2022H059)。

摘　　要：目的观察芩百清肺浓缩丸(芩百)对感染后咳嗽(PIC)大鼠肿瘤坏死因子-α(TNF-α)/核苷酸结合寡聚化结构域样受体3(NLRP3)/半胱氨酶天冬氨酶特异性蛋白酶(Caspase-1)信号通路的影响进而减轻气道高反应性。方法40只SD健康雄性大鼠随机分为空白组、模型组、芩百组、阳性组,烟熏联合LPS溶液鼻滴法及辣椒素溶液雾化吸入建立模型,观察大鼠咳嗽次数;ELISA法测定血清及支气管肺泡灌洗液(BALF)中TNF-α含量;HE染色观察肺组织病理改变;RT-qPCR及Western blotting测定肺组织中NLRP3、Caspase-1表达。结果与模型组比较,芩百组咳嗽次数显著降低(P<0.01),血清及BALF中TNF-α含量显著降低(P<0.01);支气管上皮损伤及炎性反应显著改善;肺组织中NLRP3 m RNA水平及蛋白表达均显著降低(P<0.01),Caspase-1 mRNA水平、蛋白表达显著降低(P<0.05或P<0.01)。结论芩百清肺浓缩丸能显著减轻PIC大鼠的气道高反应性,其作用机制可能与TNF-α/NLRP3/Caspase-1信号通路有关。Objective:To observe the impact of Qinbai Qingfei Concentrated Pills(Qinbai)on the TNF-α/NLRP3/Caspase-1 signaling pathway,thereby alleviating airway hyperresponsiveness in post-infectious cough(PIC)rats.Methods:40 SD healthy male rats were divided into four groups:blank control group,model group,Qinbai group,and positive control group.The models were established using a combination of smoking,LPS solution nasal drops,and capsaicin solution nebulization inhalation.Cough frequency was observed;ELISA was used to measure the content of TNF-αin serum and bronchoalveolar lavage fluid(BALF);HE staining was performed to observe pathological changes in lung tissue;RT-qPCR and Western blotting were used to measure the expression of NLRP3 and Caspase-1 in lung tissue.Results:Compared with the model group,the frequency of cough significantly decreased in the Qinbai group(P<0.01);the content of TNF-αin serum and BALF also significantly decreased(P<0.01);bronchial epithelial injury and inflammatory response were improved;the mRNA levels and protein expression of NLRP3 in lung tissue were significantly reduced(P<0.01),the mRNA level of Caspase-1 was reduced and its protein expression was significantly reduced(P<0.05 or P<0.01).Conclusion:Qinbai Qingfei Concentrated Pills can significantly reduce airway hyperresponsiveness in PIC rats,and its mechanism of action may be related to the TNF-α/NLRP3/Caspase-1 signaling pathway.

关 键 词：感染后咳嗽 气道高反应性 气道损伤 芩百清肺浓缩丸 TNF-α/NLRP3/Caspase-1信号通路 

分 类 号：R285.5[医药卫生—中药学]