基于三维微流控尘肺系统对肺泡细胞的损伤研究  

Study on Alveolar Cell Injury Based on a Three-Dimensional Microfluidic Pneumoconiosis System

作　　者：田淦 邵超杰 杨雅婕 陈天义 TIAN Gan;SHAO Chaojie;YANG Yajie;CHEN Tianyi(Anhui University of Science and Technology,Huainan 232000,China)

机构地区：[1]安徽理工大学,安徽淮南232000

出　　处：《延边大学医学学报》2025年第3期16-19,共4页Journal of Medical Science Yanbian University

基　　金：安徽理工大学研究生创新基金资助项目(编号:2023cx2154)。

摘　　要：目的:构建一种三维微流控尘肺模型,研究在机械循环拉伸条件下肺泡上皮细胞的损伤情况。方法:以聚二甲基硅氧烷(PDMS)所构建的微流体模型为载体,加入二氧化硅粒子构建尘肺模型,通过循环拉伸,模拟呼吸对肺泡细胞的机械刺激。结果:成功构建了三维微流控气动阀门系统。引入二氧化硅刺激后,显示循环机械拉伸加剧二氧化硅对肺泡上皮细胞的损伤,且N-乙酰半胱胺酸(NAC)与谷胱甘肽(GSH)能明显消除这一损伤。结论:研究强调了在体外模型的发展中重建生理相关机械拉伸的重要性,不仅对肺部疾病研究提供思路,而且为毒理学、药理学的应用和临床研究提供低成本的替代方案。Objective:To establish a three-dimensional microfluidic pneumoconiosis model and investigate the injury of human pulmonary alveolar epithelial cells(HPAEpiCs)under mechanical cyclic stretching.Methods:A polydimethylsiloxane(PDMS)-based microfluidic model was utilized as a platform.Silica particles were introduced to construct the pneumoconiosis model,and cyclic stretching was applied to simulate respiratory mechanical stimulation on alveolar cells.Results:A three-dimensional microfluidic pneumatic valve system was successfully developed.Following silica particle stimulation,the results demonstrated that mechanical cyclic stretching exacerbated silica-induced damage to alveolar epithelial cells.Notably,N-acetylcysteine(NAC)and glutathione(GSH)significantly alleviated this injury.Conclusion:This study underscores the importance of reconstructing physiologically relevant mechanical stretching in the development of in vitro models.It not only provides insights for lung disease research but also offers cost-effective alternatives for applications in toxicology,pharmacology,and clinical studies.

关 键 词：尘肺 三维微流控 肺泡细胞 损伤研究 

分 类 号：R135.2[医药卫生—劳动卫生]