贵州火龙果园杂草主要病毒检测及分析  

Detection and analysis of main viruses in the weeds of pitaya orchards in Guizhou

作　　者：李淳 安星宇 叶照春[1] 吴石平[1] 王莉爽 LI Chun;AN Xingyu;YE Zhaochun;WU Shiping;WANG Lishuang(Institute of Plant Protection,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou,550009,China)

机构地区：[1]贵州省农业科学院植物保护研究所,贵阳550006

出　　处：《中国南方果树》2025年第2期99-102,共4页South China Fruits

基　　金：贵州省科技计划项目(黔科合支撑[2021]一般238)资助。

摘　　要：为了解贵州火龙果园杂草被火龙果病毒侵染情况,采用RT-PCR法检测贵州省镇宁县火龙果园60份和罗甸县火龙果果园65份杂草样品的仙人掌X病毒等5种火龙果常见病毒的侵染情况。结果表明,镇宁县火龙果园杂草病毒检出率远高于罗甸县,镇宁县病毒检出率分别为仙人掌X病毒(CVX)75.0%,CVX-NTU 11.6%,蟹爪兰X病毒(ZyVX)15.0%,火龙果X病毒(PiVX)81.6%;罗甸县只有检出ZyVX和PiVX,检出率分别为4.6%和36.9%。两个地区共有17科35种杂草携带火龙果病毒,PiVX检出率最高,为58.4%;CVX、CVX-NTU和ZyVX检出率分别为36.0%、5.6%和9.6%,未检测出SchVX。病毒复合侵染发生普遍,检出6种类型复合侵染,占阳性样品总数的70.5%。说明贵州火龙果园杂草受多种火龙果病毒侵染,杂草可作为病毒传播的中间寄主,在防控病毒时应重视杂草病毒。To understand the situation of the weeds infected by pitaya viruses in Guizhou pitaya orchards,RT-PCR was used to detect the infection of five common viruses in 60 weeds samples from pitaya orchards in Zhenning County and 65 weeds samples from pitaya orchards in Luodian County.The results showed that the detection rate of viruses in weeds of pitaya orchards in Zhenning County was much higher than that in Luodian County.The virus detection rates in Zhenning County were cactus virus X(CVX)75.0%,CVX-NTU 11.6%,zygocactus virus X(ZyVX)15.0%,and pitaya virus X(PiVX)81.6%.Only ZyVX and PiVX were detected in the weeds of pitaya orchards in Luodian County,with detection rate of 4.6%and 36.9%,respectively.There were a total of 17 families and 36 species of weeds carrying pitaya virus in two regions,with the highest detection rate of PiVX at 58.4%.The detection rate of CVX,CVX-NTU,and ZyVX was 36%,5.6%,and 9.6%,respectively,and SchVX was not detected.The occurrence of virus co-infection was common,with a total of 6 types of co-infection detected,accounting for 70.5%of the total positive samples.It indicated that weeds in pitaya orchards in Guizhou were affected by various pitaya viruses.Weeds can serve as the intermediate host of virus transmission,and attention should be paid to weed viruses in virus prevention and control.

关 键 词：火龙果 杂草 病毒 检测 仙人掌X病毒(CVX) 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]