外源NO对南高丛蓝莓离体再生及生理生化特性影响研究  

Effect of exogenous NO on in vitro regeneration and physio-biochemical characteristics of southern highbush blueberry

作　　者：刘雯雯 刘晓风 胡连清 赵鑫 冯瑞章[1] 周万海 罗靓颖 刘雅馨 岳秦珊 LIU Wenwen;LIU Xiaofeng;HU Lianqing;ZHAO Xin;FENG Ruizhang;ZHOU Wanhai;LUO Liangying;LIU Yaxin;YUE Qinshan(Faculty of Agriculture,Forestry and Food Engineering,Yibin University,Yibin,Sichuan,644007,China;Sichuan Oil Camphor Engineering Technology Research Center,Yibin University,Yibin,Sichuan,644007,China;College of Health Industry,Sichuan Tianyi College,Deyang,Sichuan,618000,China)

机构地区：[1]宜宾学院农林与食品工程学部,四川宜宾644007 [2]宜宾学院四川省油樟工程技术研究中心,四川宜宾644007 [3]四川天一学院健康产业学院,四川德阳618000

出　　处：《中国南方果树》2025年第2期175-181,共7页South China Fruits

基　　金：宜宾市农业科技计划项目(2022NY018);宜宾市科技局高端团队项目(2021YGC03)资助。

摘　　要：以南高丛蓝莓品种戴安娜(Diana)组培苗为试材,设置0(CK)、20、50、100、150μM不同浓度一氧化氮(nitric oxide,NO)供体硝普钠(sodium nitroprusside,SNP)处理蓝莓茎段,分析培养40 d茎段基部愈伤组织的质膜透性、活性氧代谢、抗氧化产物含量和抗氧化酶活性等变化,培养70 d测定株高、茎鲜质量、茎干质量和叶绿素含量,计算分化出芽率和增殖倍数,以期探究外源NO供体SNP对南高丛蓝莓离体再生及生理生化特性的影响。结果表明:NO供体SNP对蓝莓瓶内苗的不定芽再生、增殖和生长有较明显的影响,但存在显著的浓度效应,20μM SNP处理的始分化时间最短,分化出芽率、增殖倍数和叶绿素含量最高;50μM SNP处理的鲜质量和干质量最高,150μM SNP处理的株高、叶面积最大;同时20μM SNP处理显著提高了茎段基部愈伤组织中SOD、GPX活性,增加了非酶抗氧化物ASA和GSH含量,降低了O-2产生速率和H_(2)O_(2)积累,有效缓解了膜脂过氧化作用,减轻了茎基部愈伤组织的褐化,从而促进了戴安娜茎段的分化和增殖。综上所述,在WPM+ZT 2.0 mg/L培养基中添加20μM SNP是戴安娜蓝莓不定芽再生和增殖的最佳处理。In order to clarify the effects of exogenous NO donor SNP on the in vitro regeneration and physio-biochemical properties of southern highbush blueberries,in vitro cultured seedlings of southern highbush blueberry variety Diana was used as test material,and NO donor SNP at concentrations of 0(CK),20,50,100,and 150μM were set up to treat blueberry stem segments.The changes in membrane permeability,reactive oxygen metabolism,antioxidant product contents and the antioxidant enzyme activities of callus at the base of the stem segments were analyzed after 40 days of culture.After 70 days of culture,plant height,fresh stem weight,dry stem weight,and chlorophyll content were measured,and the bud differentiation rate and proliferation multiples were calculated.The results showed that NO donor SNP had a significant effect on the regeneration,proliferation and growth of adventitious buds of blueberry seedlings cultured in bottles,but with significant concentration effect.20μM SNP treatment had the shortest initial differentiation time,with the highest differentiation rate,proliferation multiple and chlorophyll content.50μM SNP treatment resulted the highest fresh weight and dry weight,while 150μM SNP treatment had the highest plant height and largest leaf area.Meantime,20μM SNP treatment significantly increased the activities of SOD and GPX of callus in the stem base,increased the contents of non-enzymatic antioxidants ASA and GSH,reduced the production rate of O·-2 and the accumulation of H_(2)O_(2),effectively alleviated the membrane lipid peroxidation,reduced the browning of callus at stem base,and promoted the differentiation and proliferation of Diana stem segments.In summary,the addition of 20μM SNP to WPM+ZT 2.0 mg/L medium was the best treatment for the regeneration and proliferation of adventitious bud of Diana blueberry.

关 键 词：蓝莓 一氧化氮 不定芽 生长 

分 类 号：S663.9[农业科学—果树学]