载血人参石油醚部位胶束-白及多糖水凝胶对大鼠慢性创面愈合的促进作用  

Promoting effect of blood-loaded petroleum ether extract of Indigofera stachyodes micelles-Bletilla striata polysaccharide hydrogel on chronic wound healing in rats

作　　者：郑力凡 宋选飞 钟靓 肖思羽 赵婵 刘耀 徐剑 张永萍 ZHENG Lifan;SONG Xuanfei;ZHONG Liang;XIAO Siyu;ZHAO Chan;LIU Yao;XU Jian;ZHANG Yongping(College of Pharmaceutical Sciences,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;不详)

机构地区：[1]贵州中医药大学药学院,贵州贵阳550025 [2]贵州省民族药经皮给药制剂工程技术研究中心,贵州贵阳550025 [3]国家苗药工程技术研究中心,贵州贵阳550025

出　　处：《山东医药》2025年第4期21-25,共5页Shandong Medical Journal

基　　金：贵州省科技计划项目[黔科合中引地(2023)006];贵州省高层次创新型人才项目[黔科合平台人才-GCC(2023)037];贵州省教育厅滚动支持省属高校科研平台团队项目[黔教技(2022)022]。

摘　　要：目的观察载血人参石油醚部位胶束-白及多糖水凝胶(ISs-M-Gel)对大鼠慢性创面愈合的促进作用。方法80只雄性SPF级SD大鼠随机分为A、B、C、D、E组,每组16只。在各组大鼠背部制作2.5 cm×2.5 cm大小的创口,肌内注射青霉素钾(4000单位/次,1次/天,连续4 d)和醋酸氢化可的松(80 mg/kg,1次/天,连续7 d),以制备慢性创面模型。制模成功后,A组大鼠创面涂抹纯水,B组大鼠创面涂抹牛碱性成纤维细胞生长因子外用溶液,C组大鼠创面涂抹血人参石油醚部位(ISs),D组大鼠创面涂抹空白胶束-白及多糖水凝胶(Blank-M-Gel),E组大鼠创面涂抹ISs-M-Gel,每日涂抹1次,连续21 d。拍摄干预第3、7、14、21天的创面,计算创面愈合率;在上述时间点选取各组大鼠,取血制备血清,采用酶联免疫吸附试验检测血清白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α),并取创面再生皮肤组织进行HE、Masson染色,另采用总超氧化物歧化酶(SOD)和丙二醛(MDA)试剂盒检测创面再生皮肤组织SOD和MDA含量,使用免疫组化法检测创面再生皮肤组织CD31,以评估血管生成情况。结果干预第14、21天,与A组比较,其余各组创面愈合率均提高(P均<0.05);与A组比较,干预第7、14天B组和E组大鼠血清IL-6与TNF-α水平降低,干预第14、21天B组和E组创面再生皮肤组织SOD升高而MDA降低(P均<0.05)。与其他给药组比较,B组与E组再生皮肤纤维组织沉积更密集。干预第14、21天,与A组比较,B、C、E组大鼠创面再生皮肤组织CD31阳性表达增多,血管生成情况最好。结论ISs-M-Gel可促进大鼠慢性创面愈合,且效果好于ISs、Blank-M-Gel,与牛碱性成纤维细胞生长因子外用溶液效果相当。Objective To observe the promoting of blood-loaded petroleum ether extract of Indigofera stachyodes mi-celles-Bletilla striata polysaccharide hydrogel(ISs-M-Gel)on chronic wound healing in rats.Methods Eighty male SPF SD rats were randomly divided into groups A,B,C,D,and E,with 16 rats in each group.A 2.5 cm×2.5 cm wound was made on the back of rats in each group.The chronic wound model was prepared by intramuscular injection of penicillin po-tassium(4,000 units/day,once a day,for four consecutive days)and hydrocortisone acetate(80 mg/kg,once a day,for seven consecutive days).After the chronic wound models of rats were successfully constructed,the chronic wound in the group A was applied with pure water,the chronic wound in the group B was applied with bovine basic fibroblast growth fac-tor external solution,the chronic wound in the group C was applied with the petroleum ether extract of Indigofera stachyo-des(ISs),the chronic wound in the group D was applied with blank micelle-Bletilla striata polysaccharide hydrogel(Blank-M-Gel),and the chronic wound in the group E was applied with ISs-M-Gel.The wounds on the 3rd,7th,14th,and 21st days of treatment were photographed,and the wound healing rate was calculated.At the above time point,the blood of rats in each group was collected to prepare serum,serum interleukin-6(IL-6)and tumor necrosis factorα(TNF-α)were detected by enzyme-linked immunosorbent assay,and wound regenerated skin tissues were obtained for HE and Mas-son staining.Total superoxide dismutase(SOD)and malondialdehyde(MDA)kits were used to detect SOD and MDA con-tent in wound-regenerated skin tissues.Immunohistochemical method was used to detect platelet/endothelial cell adhesion molecule-1(CD31)in wound-regenerated skin tissues to evaluate angiogenesis.Results On the 14th and 21st days of intervention,compared with the group A,the wound healing rate in the other groups increased(P<0.05).On the 7th and 14th days of intervention,compared with the group A,the serum levels of IL-6 and TNF-αin the groups

关 键 词：载血人参石油醚部位胶束-白及多糖水凝胶 牛碱性成纤维细胞生长因子外用溶液 慢性创面 创面愈合 

分 类 号：R285.5[医药卫生—中药学]