精子DNA碎片率与其他精液参数的相关性分析  

Correlation Analysis of Sperm DNA Fragmentation Rate with Other Semen Parameters

作　　者：苗德雨 王祖龙[1] 王晨 MIAO Deyu;WANG Zulong;WANG Chen(Department of Andrology,the First Affiliated Hospital to Henan University of Chinese Medicine,Zhengzhou Henan China 450000;The First Clinical Medical School of Henan University of Chinese Medicine,Zhengzhou Henan China 450000;Kaifeng Hospital of Traditional Chinese Medicine,Kaifeng Henan China 475000)

机构地区：[1]河南中医药大学第一附属医院男科,河南郑州450000 [2]河南中医药大学第一临床医学院,河南郑州450000 [3]开封市中医院,河南开封475000

出　　处：《中医学报》2025年第5期919-923,共5页Acta Chinese Medicine

基　　金：国家自然科学基金项目(82174377);开封市级科技研发计划联合基金项目(2307006)。

摘　　要：目的:分析精子DNA碎片率与其他精液检查参数的相关性。方法:收集2024年2月至2024年7月就诊于河南中医药大学第一附属医院男科行精子DNA碎片率检测的患者临床相关资料及精液相关检查结果,回顾性分析精子DNA碎片率与其他数据的相关性。结果:共收集到患者信息390例,精子DNA碎片率数据390例、年龄数据390例、精液体积数据387例、精液pH数据390例、精液液化情况数据390例、精子浓度数据390例、精子前向运动百分率数据390例、精子非前向运动百分率数据388例、精子不动百分率数据388例、正常精子百分率数据374例、顶体酶活性数据182例、抗精子膜抗体数据268例;其中年龄与精子DNA碎片率呈正相关(P=0.001,r=0.173)、精液体积与精子DNA碎片率无明显线性关系(P=0.490,r=0.035)、精液pH与精子DNA碎片率无明显线性关系(P=0.623,r=-0.250)、精液液化情况与精子DNA碎片率无明显线性关系(P=0.426,r=-0.040)、精子浓度与精子DNA碎片率无明显线性关系(P=0.347,r=-0.480)、精子前向运动百分率与精子DNA碎片率呈中度负相关(P<0.001,r=-0.489)、精子非前向运动百分率与精子DNA碎片率呈低度负相关(P<0.001,r=-0.301)、精子不动百分率与精子DNA碎片率呈中度正相关(P<0.001,r=0.510)、正常精子百分率与精子DNA碎片率呈低度负相关(P<0.001,r=-0.350)、顶体酶活性与精子DNA碎片率呈低度负相关(P<0.001,r=-0.337)、抗精子膜抗体与精子DNA碎片率无明显线性关系(P=0.792,r=0.016)。结论:在诊疗精子前向运动百分率较低而精子不动百分率较高、正常精子比例较低的高龄不育患者时,建议将精子DNA碎片率检测纳入常规筛查。在精子DNA碎片率过高的不育患者诊疗过程中,可以参考精子前向运动百分率、精子非前向运动百分率、精子不动百分率和正常精子百分率推测精子DNA碎片率的变化趋势,减少精子DNA碎片率的检测频率,从而减少�Objective:to analyze the correlation between sperm DNA fragmentation rate and other semen examination parameters.Methods:Clinical data and semen-related examination results of patients who attended the Department of Men′s Medicine of the First Affiliated Hospital of Henan University of Traditional Chinese Medicine for sperm DNA fragmentation rate testing from February 2024 to July 2024 were collected,and the correlation between sperm DNA fragmentation rate and other data was analyzed retrospectively.Results:A total of 390 cases of patients were collected,390 cases of sperm DNA fragmentation rate,390 cases of age,387 cases of semen volume,390 cases of semen pH,390 cases of semen liquefaction,390 cases of sperm concentration,390 cases of sperm forward motility,390 cases of sperm non-forward motility,388 cases of sperm non-forward motility percentage data,388 cases of sperm immobility percentage data,374 cases of normal sperm percentage data,182 cases of acrosomal enzyme activity data,and 268 cases of anti-sperm membrane antibody data;among them,age showed a weak positive correlation with sperm DNA fragmentation rate(P=0.001,r=0.173),there was no significant linear correlation between semen volume and sperm DNA fragmentation rate(P=0.490,r=0.035),and there was no significant linear correlation between semen pH and sperm DNA fragmentation rate.fragmentation rate(P=0.623,r=-0.250),semen liquefaction and sperm DNA fragmentation rate(P=0.426,r=-0.040),sperm concentration and sperm DNA fragmentation rate(P=0.347,r=-0.480),and sperm forward motility percentage showed a moderate negative correlation with sperm DNA fragmentation rate(P<0.001,r=-0.489),low negative correlation between sperm non-forward motility percentage and sperm DNA fragmentation rate(P<0.001,r=-0.301),moderate positive correlation between sperm immobility percentage and sperm DNA fragmentation rate(P<0.001,r=0.510),and low negative correlation between normal sperm percentage and sperm DNA fragmentation rate(P<0.001,r=-0.350),acrosomal enzyme activity s

关 键 词：精子DNA碎片率 DNA碎片指数 男性不育症 顶体酶活性 抗精子膜抗体 精子运动 

分 类 号：R277.598.2[医药卫生—中医学]