心脉康对动脉粥样硬化小鼠主动脉组织ICAM-1、VCAM-1表达的影响  

Effect of Xinmaikang Pill on Expression of ICAM-1 and VCAM-1 in Aortic Tissue of Atherosclerotic Mice

作　　者：唐璐仪 吕洪雪 王婷[2] 叶小汉 TANG Luyi;LYU Hongxue;WANG Ting;YE Xiaohan(Guangzhou University of Chinese Medicine,Guangzhou Guangdong China 510006;Dongguan Hospital,Guangzhou University of Chinese Medicine,Dongguan Guangdong China 523000)

机构地区：[1]广州中医药大学,广东广州510006 [2]广州中医药大学东莞医院,广东东莞523000

出　　处：《中医学报》2025年第5期1085-1090,共6页Acta Chinese Medicine

基　　金：广东省粤莞青年基金项目(2020A1515110132);东莞市社会发展科技项目(20211800904402)。

摘　　要：目的:观察心脉康治疗小鼠动脉粥样硬化的效果,以及对主动脉组织细胞间黏附分子-1(intercellular cell adhesion molecule-1,ICAM-1)、血管细胞黏附因子-1(vascular cell adhesion molecule-1,VCAM-1)表达的影响。方法:将32只ApoE^(-/-)小鼠随机分为模型组、心脉康低剂量组(7 g·kg^(-1))、心脉康高剂量组(28 g·kg^(-1))及阿托伐他汀组(2.6 mg·kg^(-1)),每组8只,并以高脂饲料喂养6周。8只C57BL/6小鼠作为空白组,予普通饲料喂养6周。所有小鼠以药物或蒸馏水灌胃12周。采用HE染色检测小鼠主动脉病理形态,全自动生化分析仪检测血清中甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)、高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)浓度,ELISA法检测血清中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、氧化型低密度脂蛋白(oxidized low-density lipoprotein,ox-LDL)浓度,Western blot和qPCR法检测ICAM-1、VCAM-1蛋白与基因表达量。结果:HE染色显示,与空白组比较,模型组小鼠主动脉内斑块面积占比增加(P<0.01)。与模型组比较,阿托伐他汀组和心脉康高剂量组小鼠主动脉内斑块面积占比减少(P<0.01)。与空白组比较,模型组小鼠血清TC、TG、LDL-C、TNF-α、ox-LDL浓度升高,HDL-C浓度降低(P<0.01)。与模型组比较,心脉康高剂量组和阿托伐他汀组小鼠血清TC、TG、LDL-C、TNF-α、ox-LDL浓度降低,HDL-C浓度升高(P<0.01)。与空白组比较,模型组小鼠主动脉ICAM-1、VCAM-1蛋白和基因相对表达量升高(P<0.01)。与模型组比较,心脉康高剂量组和阿托伐他汀组小鼠主动脉ICAM-1、VCAM-1蛋白和基因相对表达量降低(P<0.01)。结论:心脉康可能通过干预主动脉ICAM-1、VCAM-1表达,从而发挥抗动脉粥样硬化的作用。Objective:To observe the effect of Xinmaikang Pill in treatment of atherosclerosis in mice,and its effect on the expression of intercellular cell adhesion molecule-1(ICAM-1)and intercellular cell adhesion factor-1(VCAM-1)in aortic tissue.Methods:Thirty-six ApoE^(-/-)mice were randomly divided into model group,Xinmaikang Pill low-dose group(7 g·kg^(-1)),Xinmaikang Pill high-dose group(28 g·kg^(-1))and atorvastatin group(2.6 mg·kg^(-1)),with 9 mice in each group,and fed with high-fat diet for 6 weeks.Nine C57BL/6 mice were treated as the blank group and fed with normal diet for 6 weeks.All mice were gavaged with drug or distilled water for 12 weeks.HE staining was used to detect the pathological morphology of the aorta in mice,and triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C)and high-density lipoprotein cholesterol were detected by automatic biochemical instrument cholesterol,HDL-C)concentration,tumor necrosis factor-α(TNF-α)and oxidized low-density lipoprotein(ox-LDL)concentrations were detected in serum by ELISA,and ICAM-1 and VCAM-1 protein and gene expressions were detected by Western blot and qPCR.Results:HE staining showed that compared with the blank group,the proportion of plaque area in the aorta in the model group increased(P<0.01).Compared with the model group,the proportion of plaque area in the aorta in the atorvastatin group and Xinmaikang Pill high-dose group was reduced(P<0.01).Compared with the blank group,the serum concentrations of TC,TG,LDL-C,TNF-αand ox-LDL in the model group increased,and the concentration of HDL-C decreased(P<0.01).Compared with the model group,the serum concentrations of TC,TG,LDL-C,TNF-αand ox-LDL in the high-dose and atorvastatin groups decreased and the concentrations of HDL-C increased(P<0.01).Compared with the blank group,the relative expressions of ICAM-1 and VCAM-1 proteins and genes in the model group were increased(P<0.01).Compared with the model group,the relative expressions of ICAM-1 and VCAM-1 proteins and genes in aort

关 键 词：心脉康 动脉粥样硬化 主动脉 ICAM-1 VCAM-1 血脂 

分 类 号：R285.5[医药卫生—中药学]