不同干燥工艺牡丹皮提取物高效液相指纹图谱建立及其抗氧化作用研究  

Development of HPLC Fingerprint and Evaluation of Antioxidant Activity in Moutan Cortex Extracts Processed by Different Drying Methods

作　　者：周芳 牛壮伟 刘淇 贾小晴 袁继承 杨纯国 褚宏伟 ZHOU Fang;NIU Zhuangwei;LIU Qi;JIA Xiaoqing;YUAN Jicheng;YANG Chunguo;CHU Hongwei(Department of Traditional Chinese Medicine,Heze Medical College,Heze 274000,China;Shandong Yifang Pharmaceutical Co.,Ltd.,Linyi 276000,China;College of Chemistry and Chemical Engineering,Hunan Normal University,Changsha 410081,China)

机构地区：[1]菏泽医学专科学校中医药系,菏泽274000 [2]山东一方制药有限公司,临沂276000 [3]湖南师范大学化学化工学院,长沙410081

出　　处：《世界中医药》2025年第3期393-398,共6页World Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(22304053);山东省中医药科技项目(2021Q131)。

摘　　要：目的:研究不同干燥工艺对牡丹皮提取物中丹皮酚、芍药苷的含量及其抗氧化作用的影响。方法:采用减压干燥、烘干和冷冻干燥对4个批次牡丹皮水提液进行干燥;高效液相色谱(HPLC)法测定各提取物中丹皮酚和芍药苷含量并建立指纹图谱;测定3种干燥工艺下牡丹皮提取物的2,2′-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)和1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除率。结果:3种干燥工艺下牡丹皮提取物中丹皮酚含量为0.0188~1.4789μg/mL,且含量从高到低依次为冷冻干燥>烘干>减压干燥;芍药苷含量为0.7301~1.2129μg/mL。12个批次样品的相似度为0.812~0.973;不同干燥工艺下提取物(质量浓度1000μg/mL)的ABTS自由基清除率为52.45%~78.64%,且冷冻干燥>烘干>减压干燥;不同干燥工艺对提取物(质量浓度1000μg/mL)的DPPH自由基清除率为51.48%~64.37%。结论:不同干燥工艺对牡丹皮提取物中丹皮酚含量和ABTS自由基清除能力影响较大。采用冷冻干燥法替代传统干燥工艺能减少丹皮酚含量损失,提高抗氧化能力,可为牡丹皮及其制剂开发提供参考。Objective:To investigate the effects of different drying methods on the content of paeonol and paeoniflorin in Moutan Cortex extracts and its antioxidant activity.Methods:Four batches of aqueous extracts of Moutan Cortex were subjected to vacuum drying,oven drying,and freeze drying.The content of paeonol and paeoniflorin in each extract was determined using high-performance liquid chromatography(HPLC),and fingerprint analysis was established.The antioxidant activity of the extracts obtained from the three drying methods was evaluated based on their 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)and 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical scavenging capacities.Results:The paeonol content in the extracts ranged from 0.0188 to 1.4789μg/mL,with the highest content observed in freeze-dried samples,followed by oven drying and vacuum drying.The paeoniflorin content ranged from 0.7301 to 1.2129μg/mL.The sample similarity in the 12 batches ranged from 0.812 to 0.973.At a concentration of 1000μg/mL,the ABTS radical scavenging rate of the extracts ranged from 52.45%to 78.64%,following the order freeze drying>oven drying>vacuum drying.The DPPH radical scavenging rate ranged from 51.48%to 64.37%across different drying methods.Conclusion:Different drying methods significantly affect the paeonol content and ABTS radical scavenging capacity of Moutan Cortex extracts.Freeze drying is recommended as an alternative to traditional drying methods,as it reduces paeonol loss and enhances antioxidant activity,providing a reference for the development of Moutan Cortex and its preparations.

关 键 词：牡丹皮 丹皮酚 芍药苷 抗氧化 冷冻干燥 干燥工艺 指纹图谱 

分 类 号：R298[医药卫生—民族医学] R284.1[医药卫生—中医学]