黄连制吴茱萸炮制前后抗炎、抗氧化、抑菌作用研究  

Anti-Inflammatory,Anti-Oxidation,and Anti-Bacterial Effects of Evodiae Fructus Before and After Processing with Coptidis Rhizoma

作　　者：刘舒凌[1] 李秀连 梁灵南 刘玉兰 廖美东 吴燕春[1] LIU Shuling;LI Xiulian;LIANG Lingnan;LIU Yulan;LIAO Meidong;WU Yanchun(Guangxi University of Chinese Medicine,Nanning,Guangxi,China 530200)

机构地区：[1]广西中医药大学,广西南宁530200

出　　处：《中国药业》2025年第9期34-39,共6页China Pharmaceuticals

基　　金：广西自然科学基金[2025GXNSFAA069558,2018GXNSFBA281179];2022年中央补助广西中药炮制技术传承基地建设资助项目[桂中医药发〔2022〕9号];广西中医药大学校级科研项目[2023MS019]。

摘　　要：目的探讨吴茱萸经黄连炮制前后的抗炎、抗氧化及抑菌作用。方法制备净制吴茱萸饮片、黄连制吴茱萸饮片。采用二甲苯致小鼠耳肿胀模型评价体内抗炎作用及对脏器指数的影响,将66只KM小鼠随机分为空白对照组(A组,等体积生理盐水),阳性药对照组(B组,醋酸地塞米松5 mg/kg),吴茱萸高、低剂量组(C_(1)组、C_(2)组,5,1 g/kg),黄连制吴茱萸高、低剂量组(D_(1)组、D_(2)组,5,1 g/kg),各11只,灌胃相应药物或20 mL/kg生理盐水,每日1次,连续5 d。采用脂多糖(LPS)诱导小鼠单核巨噬细胞(RAW 264.7)炎性反应,检测空白对照(G组,接种细胞,含等体积DMEM培养液)及不同质量浓度(4,2,1,0.5,0.25 mg/mL)吴茱萸(E1组、E2组、E3组、E4组、E5组)和黄连制吴茱萸(F_(1)组、F_(2)组、F_(3)组、F_(4)组、F_(5)组)对细胞存活率的影响;比较空白组(H组,1 mLDMEM培养液),模型组(I组,含1μg/mLLPS的DMEM培养液1 mL),吴茱萸高、中、低剂量组(J_(1)组、J_(2)组、J_(3)组,含2,1,0.5 mg/mL药液和1μg/mLLPS的DMEM培养液1 mL),黄连制吴茱萸高、中、低剂量组(K_(1)组、K_(2)组、K_(3)组,含2,1,0.5 mg/mL药液和1μg/mLLPS的DMEM培养液1 mL)一氧化氮(NO)、白细胞介素6(IL-6)、肿瘤坏死因子-α(TNF-α)的水平。采用1,1-二苯基-2-三硝基苯肼(DPPH)评价体外抗氧化活性,采用纸片法和微量二倍稀释法评价对金黄色葡萄球菌和大肠杆菌的体外抑菌活性。结果与A组比较,B组、C_(1)组、C_(2)组、D_(1)组、D_(2)组小鼠耳肿胀度均显著降低(P<0.05),B组、D_(1)组、D_(2)组小鼠胸腺指数均显著降低(P<0.01),B组、C_(1)组、D_(1)组、D_(2)组小鼠脾指数均显著降低(P<0.05),且D_(1)组、D_(2)组小鼠以上指标分别显著低于C_(1)组、C_(2)组(P<0.05)。与G组比较,E1组和F_(1)组的吸光度均显著降低(P<0.01),E5组的吸光度显著升高(P<0.05)。与I组比较,J_(1)组、J_(2)组、J_(3)组、K_(1)组、K_(2)组、K_(3)组NO水平均显著降低(P<0.0Objective To investigate the anti-inflammatory,anti-oxidation and anti-bacterial effects of Evodiae Fructus before and after processing with Coptidis Rhizoma.Methods Products of Evodiae Fructus(EF)and Evodiae Fructus processed with Coptidis Rhizoma(EFCR)were prepared.The anti-inflammatory effect in vivo and its effect on organ indexes were evaluated by the xylene-induced ear edema model in mice.A total of 66 KM mice were randomly divided into the blank control group(group A,equal volume of normal saline),the positive drug control group(group B,5 mg/kg dexamethasone acetate),the EF high-and low-dose groups(groups C_(1),C_(2);5,1 g/kg),and the EFCR high-dose and low-dose group(groups D_(1),D_(2);5,1 g/kg),with 11 mice in each group.The mice in each group were orally administered with the corresponding drug or 20 mL/kg normal saline once a day for 5 d.Lipopolysaccharide(LPS)was used to induce inflammatory reaction in mice mononuclear macrophages(RAW 264.7),and the effects of the blank control(group G,inoculated cells,containing equal volume of DMEM culture medium)and different mass concentrations(4,2,1,0.5,0.25 mg/mL)of EF(groups E1,E2,E3,E4,E5)and EFCR(groups F_(1),F_(2),F_(3),F_(4),F_(5))on cell survival rate were detected.The levels of nitric oxide(NO),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α)in the blank group(group H,1 mL of DMEM culture medium),model group(group I,1 mL of DMEM culture medium containing 1µg/mL LPS),EF high-,medium-,and low-dose groups(groups J_(1),J_(2),J_(3);1 mL of DMEM culture medium containing 1,0.5,0.25 mg/mL liquid medicine,and 1µg/mL LPS),and EFCR high-,medium-,and low-dose groups(groups K_(1),K_(2),K 3;1 mL DMEM culture medium containing 1,0.5,0.25 mg/mL liquid medicine,and 1µg/mL LPS)were compared.The in vitro anti-oxidant activity was evaluated by 1,1-diphenyl-2-picrylhydazy(DPPH),and the in vitro anti-bacterial activity against Staphylococcus aureus and Escherichia coli was evaluated by the disk diffusion method and micro double dilution method.Results Compared with

关 键 词：黄连制吴茱萸 炮制 抗炎作用 抗氧化作用 抑菌作用 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学] R286.0