除湿化毒方治疗湿疹有效成分及作用机制研究  

Effective Components and Mechanism of Dehumidification and Detoxification Formula in the Treatment of Eczema

作　　者：李博 石惠云 杨绘琳 杨秋雨 邱洪 LI Bo;SHI Huiyun;YANG Huilin;YANG Qiuyu;QIU Hong(The 925th Hospital of the Chinese People's Liberation Army Joint Logistic Support Force,Guiyang,Guizhou,China 550000)

机构地区：[1]中国人民解放军联勤保障部队第九二五医院,贵州贵阳550000

出　　处：《中国药业》2025年第9期46-52,共7页China Pharmaceuticals

基　　金：贵州省中医药管理局中医药、民族医药科学技术研究课题[QZYY-2023-128]。

摘　　要：目的探讨除湿化毒方治疗湿疹的有效成分及作用机制。方法通过TCMSP数据库筛选除湿化毒方的活性成分,通过Drug⁃Bank数据库筛选活性成分相关靶点,通过GeneCards数据库筛选湿疹相关靶点,通过Python 3.8版编程软件筛选除湿化毒方活性成分和湿疹的共有靶点,通过Cytoscape 3.6.0软件构建药物-成分-疾病-靶点网络,通过String数据库进行蛋白-蛋白互作(PPI)分析,通过DAVID数据库进行基因本体论(GO)功能分析、京都基因与基因组百科全书(KEGG)通路富集分析。将50只BALB/C小鼠随机分为空白对照组(A组),模型对照组(B组),阳性药对照组(C组),除湿化毒方低、高浓度组(D_(1)组、D_(2)组),各10只。腹部涂抹5%2,4-二硝基氯苯(DNCB)致敏2次+1%DNCB激发4次,以复制湿疹小鼠模型。建模成功后,C组小鼠耳部外涂醋酸地塞米松乳膏(规格为每支30 g∶22.5 mg)0.1 g,D_(1)组、D_(2)组小鼠耳部分别外涂45,180 mg/mL除湿化毒方药液5 mL,A组、B组小鼠不做特殊处理,连续给药12 d。测量小鼠耳部质量,苏木精-伊红(HE)染色观察耳部组织形态,采用酶联免疫吸附试验(ELISA)法检测小鼠血清中肿瘤坏死因子-α(TNF-α)、白细胞介素6(IL-6)、白细胞介素2(IL-2)的表达水平,采用免疫组化法检测小鼠耳部组织TNF-α,IL-6,IL-2的蛋白表达水平。结果共筛选出144种活性成分,41个共有靶点,核心活性成分有槲皮素、木犀草素、山柰酚、黄芩素等,核心靶点有β2肾上腺素能受体(ADRB2)、丝氨酸-苏氨酸蛋白激酶1(Akt1)、一氧化氮合酶(NOS2)、TNF、IL-6、IL-2等,涉及TNF,IL-2,IL-17等信号通路。与B组比较,D_(1)组、D_(2)组小鼠耳部炎性细胞浸润及水肿现象减轻,皮肤病理变化改善;耳部质量差及血清右耳组织中TNF-α,IL-6,IL-2表达水平均显著降低(P<0.05),且上述指标的改善效果均以D_(2)组更佳;D_(2)组小鼠右耳组织中TNF-α,IL-6,IL-2蛋白表达水平均显著降低(P<0.05)。结论网Objective To investigate the effective components and mechanism of Dehumidification and Detoxification Formula in the treatment of eczema.Methods The active components of Dehumidification and Detoxification Formula were screened by the TCMSP database,the active component-related targets were screened by the DrugBank database,eczema-related targets were screened by the GeneCards database,the active components of Dehumidification and Detoxification Formula and common targets of eczema were screened by the Python 3.8 programming software,the drug-component-disease target network was constructed by the Cytoscape 3.6.0 software,the protein-protein interaction(PPI)analysis was performed by the String database,and the Gene Ontology(GO)functional analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed by the DAVID database.A total of 50 BALB/C mice were randomly divided into the blank control group(group A),the model control group(group B),the positive drug control group(group C),and the Dehumidification and Detoxification Formula low-and high-dose groups(groups D_(1) and D_(2)),with 10 mice in each group.The 5%2,4-dinitrochlorobenzene(DNCB)was applied to the abdomen of mice for sensitization twice and 1%DNCB was applied for stimulation four times to replicate the eczema model mice.After successful modeling,0.1 g of Dexamethasone Acetate Cream(30 g∶22.5 mg)was applied externally to the ears of mice in group C,and 5 mL of Dehumidification and Detoxification Formula(45 mg/mL and 180 mg/mL)were applied externally to the ears of mice in groups D_(1) and D_(2),respectively.Mice in groups A and B did not receive any special treatment.All mice were continuously administered for 12 d.The mass of mouse ear was weighted,and the morphology of ear tissue was observed by the hematoxylin-eosin(HE)staining.Enzyme-linked immunosorbent assay(ELISA)was used to detect the expression levels of serum tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and interleukin-2(IL-2).Immunohistochemistr

关 键 词：湿疹 除湿化毒方 网络药理学 动物实验 有效成分 作用机制 

分 类 号：R932[医药卫生—生药学] R285.5[医药卫生—药学]