Bacterial abundance and diversity in 64–74 Ma subseafloor igneous basement from the Louisville Seamount Chain  

作　　者：Jason B.Sylvan Benjamin J.Tully Yuki Morono Jeffrey C.Alt Sharon L.Grim Fumio Inagaki Anthony A.P.Koppers Katrina J.Edwards 

机构地区：[1]Department of Biological Sciences,University of Southern California,Los Angeles,California,USA [2]Center for Dark Energy Biosphere Investigations,University of Southern California,Los Angeles,California,USA [3]Kochi Institute for Core Sample Research,Japan Agency for Earth‐Marine Science and Technology(JAMSTEC),Nankoku,Kochi,Japan [4]Department of Earth and Environmental Sciences,University of Michigan,Ann Arbor,Michigan,USA [5]Josephine Bay Paul Center for Comparative Molecular Biology and Evolution,Marine Biological Laboratory,Woods Hole,Massachusetts,USA [6]Advanced Institute for Marine Ecosystem Change(WPI‐AIMEC),JAMSTEC,Yokohama,Japan [7]Department of Earth Sciences,Graduate School of Science,Tohoku University,Sendai,Japan [8]College of Earth,Ocean and Atmospheric Sciences,Oregon State University,Corvallis,Oregon,USA [9]Department of Oceanography,Texas A&M University,College Station,Texas,USA [10]Department of Marine Chemistry and Geochemistry,Woods Hole Oceanographic Institution,Woods Hole,Massachusetts,USA

出　　处：《mLife》2024年第4期578-583,共6页微生物(英文)

基　　金：funded by grant number T330A55 from the Consortium for Ocean Leadership to JBS and KJE;a postdoctoral fellowship from the Center for Dark Energy Biosphere Investigations(C‐DEBI)to JBS;a grant to JBS from C‐DEBI to support cell counts conducted at the Kochi Core Center;NSF Science and Technology Center grant number 0939564 to KJE that also supported BJT;Texas A&M University startup funds to JBS.

摘　　要：Impact statement The aquifer in the subseafloor igneous basement is a massive,continuous microbial substrate,yet sparingly little is known about life in this habitat.The work to date has focused largely on describing microbial diversity in the young basement(<10 Ma),where the basaltic crust is still porous and fluid flow through it is active.Here,we test the hypothesis that microbial life exists in subseafloor basement>65 Ma using samples collected from the Louisville Seamount Chain via seafloor drilling.Cell biomass was heterogeneous in nature,ranging from below detection to~104 cells cm−3.Bacterial 16S rRNA genes from core samples and enrichment incubations are dominated by lineages putatively carrying out nitrogen,sulfur,and metal redox processes and hydrocarbon oxidation.Taken together,the data indicate that microbial life is indeed present in subseafloor igneous basement>65 Ma,which significantly expands the range of the subseafloor biosphere where microbial life is known to exist.

关 键 词：basaltic crust bacterial abundance fluid flow subseafloor igneous basement seafloor drillingcell microbial diversity Louisville Seamount Chain 

分 类 号：Q938[生物学—微生物学]