康复运动通过调节大鼠肺脏NADPH氧化酶延缓慢性阻塞性肺疾病进程的研究  

A study on rehabilitation exercise delaying the progression of chronic obstructive pulmonary disease by regulating NADPH oxidase in lung

作　　者：孙维梁 郭静[1] 张雨婷 曲木诗玮 SUN Weiliang;GUO Jing;ZHANG Yuting(China-Japan Friendship Hospital,Beijing,100029)

机构地区：[1]中日友好医院临床医学研究所,北京市100029 [2]中日友好医院呼吸与危重症医学科

出　　处：《中国康复医学杂志》2025年第4期495-500,共6页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金青年项目(82200051)。

摘　　要：目的:探讨NADPH氧化酶(NADPH oxidase,Nox)分子亚型在康复运动改善慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)中的表达及机制。方法:将大鼠随机分为对照组、COPD组、COPD中等强度运动组和COPD较大强度运动组,每组10只。免疫组化检测缺氧诱导因子1α(hypoxia inducible factor-1α,HIF1α)、NADPH氧化酶分子、炎症因子——肿瘤坏死因子α(factors tumor necrosis factor alpha,TNFα)、白细胞介素-6(interleukin-6,IL-6)、基质金属蛋白酶2(matrix metalloproteinase 2,MMP-2)和基质金属蛋白酶9(matrix metalloproteinase 9,MMP-9)的表达。结果:与对照组相比,COPD组NADPH氧化酶亚型Nox1、Nox2、Nox5、HIF1α、TNFα、IL-6、MMP-2和MMP-9表达明显增加,中等强度运动可以减少COPD动物模型Nox2、Nox5、MMP-9、HIF1α表达,较大强度运动可以减少Nox1、Nox2、Nox5、TNFα、IL-6、MMP-9、HIF1α表达。结论:康复运动下调肺脏NADPH氧化酶不同亚型分子表达,进而减少下游炎症因子、TNFα、IL-6、MMP-2和MMP-9的表达,康复运动的保护功能可能通过抑制HIF1α的表达而实现。Objective:To investigate the expression of NADPH oxidase(Nox)in chronic obstructive pulmonary disease(COPD).Method:The rats were randomly divided into control group,COPD group,COPD medium exercise group and COPD intensive exercise group,with 10 rats in each group.The expression of NADPH oxidase,hypoxia inducible factor-1 alpha(HIF1α),inflammatory factors tumor necrosis——factor alpha(TNFα),interleukin-6(IL-6),matrix metalloproteinase 2(MMP-2)and matrix metalloproteinase 9(MMP-9)were detected by immunohistochemistry.Result:Compared with the control group,the expression of NADPH oxidase subtypes Nox1,Nox2,Nox5,HIF1α,TNFα,IL-6,MMP-2 and MMP-9 in COPD group increased significantly.Medium exercise reduced the expression of Nox2,Nox5,HIF1αand MMP-9 in COPD animal model,while intensive exercise reduced the expression of Nox1,Nox2,Nox5,HIF1α,TNFα,IL-6 and MMP-9.Conclusion:Rehabilitation exercise down regulated the expression of NADPH oxidases in lung,reduced the expression of downstream inflammatory factors and matrix metalloproteinase.The protective function of rehabilitation exercise might achieve by inhibiting the expression of hypoxia inducible factor.

关 键 词：慢性阻塞性肺疾病 NADPH氧化酶 缺氧诱导因子 炎症因子 基质金属蛋白酶 

分 类 号：R493[医药卫生—康复医学] R563.9[医药卫生—临床医学]