以鲁米诺/过氧化氢作为报告体系的微通道电化学发光传感器用于四环素的检测  

Microchannel-based Electrochemiluminescence Sensor for Tetracycline Detection Using Luminol/Hydrogen Peroxide as Reporter System

作　　者：黄少坤 谢秀琳 蔡华宾 黄艳玲 林悦 林振宇 HUANG Shao-Kun;XIE Xiu-Lin;CAI Hua-Bin;HUANG Yan-Ling;LIN Yue;LIN Zhen-Yu(Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety,Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology,College of Chemistry,Fuzhou University,Fuzhou 350116,China)

机构地区：[1]福州大学化学学院,食品安全与生物分析教育部重点实验室,福建省食品安全分析与检测重点实验室,福州350116

出　　处：《分析化学》2025年第3期356-363,共8页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.22374024);福州大学化学学院化学拔尖人才创新训练计划项目(No.HXBJ202404)资助。

摘　　要：采用鲁米诺/H_(2)O_(2)作为微通道电化学发光(ECL)报告体系,构建了一种微通道ECL传感器用于四环素(TC)的检测。鲁米诺/H_(2)O_(2) ECL体系较低的激发电位可以降低体系钳位电压的影响,从而提高微通道ECL传感器的检测性能。修饰了四环素适配体(TC-aptamer)的微通道可特异性识别并捕获TC,TC带有的正电荷导致微通道内界面负电荷密度降低,使微通道的离子电流增加,导致体系ECL信号增强。对电解质浓度、TC-aptamer修饰液浓度、TC与TC-aptamer的反应时间等条件进行了优化。在最优条件下,体系ECL信号变化值与TC浓度在1.00~200 ng/mL范围内呈良好的线性关系,检出限为0.69 ng/mL。此传感器具有良好的选择性,并成功用于牛奶样本中TC的检测。A microchannel-based electrochemiluminescence(ECL)sensor was developed for detection of tetracycline(TC)utilizing luminol/H_(2)O_(2) as ECL reporting system.The low excitation potential of luminol/H_(2)O_(2) effectively mitigated the impact of clamping voltage,thereby enhancing the detection performance of the microchannel-based ECL sensor.The microchannel modified with TC aptamer selectively recognized and captured target TC.The positively charged TC reduced the surface charge density within the microchannel,thereby increasing the ionic current in the microchannel,leading to change of ECL signal of system.The experimental conditions such as electrolyte concentration,TC-aptamer concentration,and reaction time between TC and TCaptamer were optimized.Under optimal conditions,the difference of ECL signal in the absence and presence of TC(ΔECL)exhibited a good linear relationship with TC concentration in the range from 1.00 ng/mL to 200 ng/mL,with a detection limit as low as 0.69 ng/mL.The sensor had good selectivity and was successfully used in detection of TC in milk samples.

关 键 词：电化学发光 鲁米诺 微通道 四环素 适配体 

分 类 号：O657.1[理学—分析化学]