大肠杆菌胞外多糖/万古霉素联用抗耐甲氧西林金黄色葡萄球菌生物膜性能分析  

Escherichia coli Exopolysaccharides/Vancomycin Combination for Combating Methicillin-resistant Staphylococcus aureus Biofilm

作　　者：万晨潇 鞠晓燕 田野[1] 牛忠伟[1,3] WAN Chen-Xiao;JU Xiao-Yan;TIAN Ye;NIU Zhong-Wei(Key Laboratory of Photochemical Conversion and Optoelectronic Materials,Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Beijing 100190,China;University of Chinese Academy of Sciences,Beijing 100049,China;School of Future Technology,University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院理化技术研究所,中国科学院光化学转换与功能材料重点实验室,北京100190 [2]中国科学院大学,北京100049 [3]中国科学院大学未来技术学院,北京100049

出　　处：《分析化学》2025年第3期418-428,共11页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.52073293,82204326,52273160);中国科学院理化技术研究所所长基金资助。

摘　　要：细菌生物膜的形成是导致细菌感染性疾病难以治疗的主要原因之一,因此开发有效的抗生物膜策略对于药物研发和临床治疗具有重要的意义。尽管抗生素是临床治疗细菌感染的重要手段,但在治疗与细菌生物膜相关的疾病时,其疗效通常不佳。本研究提取并纯化了大肠杆菌的胞外多糖(E.coli EPS),其能有效抑制耐甲氧西林金黄色葡萄球菌(MRSA)生物膜的形成。为了增强万古霉素(VAN)对生物膜的抑制效果,考察了E.coli EPS与VAN联合使用的效果。相比于单独使用VAN,E.coli EPS与VAN联合使用提高了对MRSA生物膜形成的抑制率,同时对成熟生物膜的分散率从10%显著提升至80%。与单独使用相同浓度的VAN相比,VAN与E.coli EPS的联合使用能够使生物膜内部细菌菌落数减少88%。此外,E.coli EPS可以显著下调MRSA生物膜相关基因的表达。本研究结果表明,E.coli EPS具有良好的抗生物膜活性,与VAN联合使用可作为MRSA生物膜感染的潜在治疗手段。Biofilm formation is one of the key reasons that bacterial infections are difficult to treat.So it is of great significance to develop effective strategies to resist bacterial biofilms.Although antibiotics are important clinical tools for the treatment of bacterial infections,their therapeutic efficacy is often unsatisfactory when targeting bacterial biofilm-associated diseases.In this study,exopolysaccharides(EPS)from Escherichia coli(E.coli)were extracted and purified.It was demonstrated that the obtained E.coli EPS had the ability to inhibit methicillinresistant Staphylococcus aureus(MRSA)biofilm formation and disperse a mature biofilm.To improve the antibiofilm effect of vancomycin(VAN),E.coli EPS was used in combination with VAN.The combination increased the inhibition rate of MRSA biofilm and increased the dispersion rate of mature biofilm from 10% to 80%.When combined with E.coli EPS,the number of bacterial colonies within the MRSA biofilm remarkably decreased by 88%,resulting in a significant improvement over the use of VAN alone at an equivalent concentration.Meanwhile,E.coli EPS could down-regulate the expression of MRSA biofilm-related genes.E.coli EPS showed good anti-biofilm effect,and E.coli EPS/VAN combination could provided a potential strategy for treatment of MRSA biofilm infections.

关 键 词：生物膜 大肠杆菌胞外多糖 耐甲氧西林金黄色葡萄球菌 抗生素 生物膜 

分 类 号：R378[医药卫生—病原生物学]