Cloning and Bioinformatics Analysis of cobQ Gene from Vibrio alginolyticus Strain HY9901  

作　　者：Chao HUANG Zhihang CHEN Weijie ZHANG Xiaoxin WEN Jiajie MA Peng ZHOU Yingying JIANG Huanying PANG 无 

机构地区：[1]Fisheries College,Guangdong Ocean University,Zhanjiang 524025,China [2]Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture&Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes,Zhanjiang 524025,China

出　　处：《Asian Agricultural Research》2025年第4期19-23,共5页亚洲农业研究(英文)

基　　金：Natural Science Foundation of Guangdong Province(2025A1515011061);Outstanding Graduate Entering Laboratory Project of College of Fisheries,Guangdong Ocean University;Undergraduate Innovation and Entrepreneurship Training Program of Guangdong Ocean University(CXXL2024007);Undergraduate Innovation Team of Guangdong Ocean University(CCTD201802).

摘　　要：[Objectives] To analyze the function of cobQ gene from Vibrio alginolyticus strain HY9901,and to provide a reference for exploring the possible mechanism of cobQ gene from V.alginolyticus.[Methods] A pair of primers were designed based on the sequence of the V.alginolyticus cobQ gene and used to amplify the full-length gene by PCR.[Results] The PCR amplification results indicated that the cobQ gene has a full length of 780 bp,encoding 259 amino acid residues.The deduced amino acid sequence predicts a molecular weight of approximately 28.83 kD and an isoelectric point of 9.21.Sequence analysis revealed no N-terminal signal peptide cleavage site,suggesting the absence of both a signal peptide and transmembrane regions in this protein.The amino acid sequence contains 2 N-terminal myristoylation sites,1 N-glycosylation site,1 glycosaminoglycan attachment site,4 microbody C-terminal targeting signal sites,3 casein kinase II phosphorylation sites,and 4 protein kinase C phosphorylation sites.Subcellular localization prediction showed that the CobQ protein is primarily localized in the cytoplasm(65.2%probability).Homology analysis demonstrated that the amino acid sequence of the cobQ gene from V.alginolyticus shares up to 99%homology with other Vibrio species,clustering within the same subclade as Vibrio parahaemolyticus,indicating close phylogenetic relationships.Secondary structure prediction revealed proportions ofα-helices,random coils,and extended strands as 44.40%,36.68%,and 18.92%,respectively.The tertiary structure model exhibited 87.62%similarity to the template A0A165XBE1.1.[Conclusions] In this study,the V.alginolyticus cobq gene was successfully cloned and its sequence was analyzed by bioinformatics.It is expected to lay a foundation for the subsequent study of the regulatory mechanism of its protein on the virulence of V.alginolyticus.

关 键 词：Vibrio alginolyticus Gene cloning cobQ gene Bioinformatics analysis 

分 类 号：S917.4[农业科学—水产科学]