复方羚角降压丸中不同比例山羊角代替羚羊角对自发性高血压大鼠的药效分析  

Efficacy of Fufang Lingjiao Jiangya Pills with Different Proportions of Goat Horn Replacing Antelope Horn on Spontaneous Hypertensive Rats

作　　者：王藤剑 赵婉璐 俞阳 刘燕 曹鲲 林哲源 吴悦 罗莉澜 赖威旨 楼招欢 张巧艳 张泉龙 秦路平 WANG Tengjian;ZHAO Wanlu;YU Yang;LIU Yan;CAO Kun;LIN Zheyuan;WU Yue;LUO Lilan;LAI Weizhi;LOU Zhaohuan;ZHANG Qiaoyan;ZHANG Quanlong;QIN Luping(School of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学药学院,杭州310053

出　　处：《中国实验方剂学杂志》2025年第9期68-78,共11页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点研发计划项目(2018YFC1706102)。

摘　　要：目的:从降血压角度,以复方羚角降压丸为例,研究复方中山羊角代替羚羊角的最佳比例及其降压作用机制。方法:在相同比例山羊角替代羚羊角复方对自发性高血压大鼠的降压药效实验中,50只SHR大鼠被随机分为模型组8只、卡托普利组(0.01 g·kg^(-1))6只、空白复方低组(0.342 g·kg^(-1))6只、空白复方高组(0.684 g·kg^(-1))6只、羚羊复方低组(0.378 g·kg^(-1))6只、羚羊复方高组(0.756 g·kg^(-1))6只,山羊复方低组(0.378 g·kg^(-1))6只、山羊复方高组(0.756 g·kg^(-1))6只,正常组为8只WKY大鼠,正常组和模型组大鼠用等体积蒸馏水灌胃,连续灌胃4周,给药期间分别在给药前、给药后3 h及每隔2周测定大鼠血压。在不同比例山羊角代替羚羊角复方对自发性高血压大鼠的降压药效实验中,48只SHR大鼠随机分为模型组、空白复方组(0.684 g·kg^(-1))、羚羊角复方组(0.756 g·kg^(-1))、2倍山羊角复方组(0.824 g·kg^(-1))、4倍山羊角复方组(0.969 g·kg^(-1))、6倍山羊角复方组(1.112 g·kg^(-1)),正常组为8只WKY大鼠,正常组和模型组大鼠用等体积蒸馏水灌胃,连续灌胃2周,给药期间分别在给药前、给药3 h、给药4 d、给药7 d、给药10 d、给药14 d测定大鼠血压。酶联免疫吸附测定法(ELISA)检测血清中血管紧张素转化酶(ACE)、血管紧张素Ⅱ(AngⅡ)、肾素(Renin)、白细胞介素-6(IL-6)含量;苏木素-伊红(HE)染色观察大鼠心脏、肾脏和胸主动脉组织形态变化;蛋白免疫印迹法(Western blot)检测大鼠肾脏组织中ACE2、血管紧张素Ⅱ受体1(AT1R)、血管紧张素原(AGT)蛋白表达;免疫组化法检测大鼠胸主动脉组织中核转录因子(NF)-κB p65、Toll样受体4(TLR4)蛋白表达。结果:与模型组比较,各复方组能明显降低大鼠血压(P<0.05,P<0.01),6倍山羊角复方组降压效果与羚羊角复方组一致。与正常组比较,模型组大鼠血清ACE、AngⅡ、Renin、IL-6水平升高,给药后各复方组�Objective:To investigate the optimal ratio of goat horn replacing antelope horn in Fufang Lingjiao Jiangya pills and the blood pressure-lowering mechanism of this medicine.Methods:The blood pressure-lowering efficacy of Fufang Lingjiao Jiangya pills with varying proportions of goat horn replacing antelope horn was evaluated on spontaneous hypertensive rats(SHR).In this experiment,50 SHR rats were randomly grouped as follows:model(n=8),captopril(0.01 g·kg^(-1))(n=6),lowdose blank Fufang Lingjiao Jiangya pills(0.342 g·kg^(-1))(n=6),high-dose blank Fufang Lingjiao Jiangya pills(0.684 g·kg^(-1))(n=6),low-dose antelope horn-containing Fufang Lingjiao Jiangya pills(0.378 g·kg^(-1))(n=6),high-dose antelope horn-containing Fufang Lingjiao Jiangya pills(0.756 g·kg^(-1))(n=6),low-dose goat horn-containing Fufang Lingjiao Jiangya pills(0.378 g·kg^(-1))(n=6),and high-dose goat horn-containing Fufang Lingjiao Jiangya pills(0.756 g·kg^(-1))(n=6).Additionally,8 WKY rats were used as the normal group.Drugs were administered by gavage for 4 weeks while an equal volume of distilled water was administered for the normal and model groups.Blood pressure was measured before administration,3 h post administration,and biweekly thereafter.In the experiment for Fufang Lingjiao Jiangya pills with goat horn replacing antelope horn in different proportions,48 SHR rats were randomly grouped as follows:model,blank Fufang Lingjiao Jiangya pills(0.684 g·kg^(-1)),antelope horn-containing Fufang Lingjiao Jiangya pills(0.756 g·kg^(-1)),2×goat horn-containing Fufang Lingjiao Jiangya pills(0.824 g·kg^(-1)),4×goat horn Fufang Lingjiao Jiangya pills(0.969 g·kg^(-1)),and 6×goat horn Fufang Lingjiao Jiangya pills(1.112 g·kg^(-1)).The normal group included 8 WKY rats,and the normal group and model group received an equal volume of distilled water.The treatment lasted for 2 weeks,and blood pressure was recorded at various time points(pre-administration,3 h post administration,and on days 4,7,10,and 14 of administration).Serum levels of angio

关 键 词：山羊角 羚羊角 复方羚角降压丸 高血压 

分 类 号：R285[医药卫生—中药学] R289[医药卫生—中医学] R544.1