基于斑马鱼模型的中药抗骨质疏松的评价方法与疗效研究进展  

Progress of Anti-osteoporosis Research of Traditional Chinese Medicine Based on Zebrafish Model

作　　者：王恒鸿 樊鑫宇 高义涵 罗智略 段鹏 周云烽 WANG Henghong;FAN Xinyu;GAO Yihan;LUO Zhilue;DUAN Peng;ZHOU Yunfeng(Xiangyang No.1 People′s Hospital,Hubei Universi ty of Medicine,Xiangyang 441000,China;Key Laboratory of Zebrafish Modeling and Drug Screening for Human Diseases of Xiangyang City,Xiangyang No.1 People's Hospital,Hubei University of Medicine,Xiangyang 441000,China)

机构地区：[1]湖北医药学院附属襄阳市第一人民医院,湖北襄阳441000 [2]湖北医药学院附属襄阳市第一人民医院人类疾病斑马鱼模型新药筛选襄阳市重点实验室,湖北襄阳441000

出　　处：《中国实验方剂学杂志》2025年第9期323-330,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：湖北省自然基金项目(2022CFB940);湖北省教育厅课题青年人才项目(Q20222108);襄阳市科技局项目(2022YL22A);湖北医药学院研究生教育教学研究项目(YJ2022018);襄阳市第一人民医院科技创新项目(XYY2023SD08)。

摘　　要：骨质疏松症(OP)是一种全身代谢性疾病,与年龄存在很大相关性。由于人口老龄化的问题日益严重,OP的发病率逐年上升。目前OP的治疗方法存在诸多缺陷,而我国传统中药历史悠久、物种丰富,近年来出现了越来越多的中药抗OP相关研究,可能为OP治疗提供安全有效的替代策略。斑马鱼因其良好的光学透明性且与人类基因高度同源,作为一种动物研究模型被广泛应用于人类骨骼相关疾病机制及药物筛选的研究中。既往相关综述对中药抗OP的相关信号通路、各种斑马鱼OP模型造模方法比较及与传统动物模型比较较少涉及,该文即通过对以斑马鱼为模型的中药抗OP研究进行综述,概括在此类研究中涉及的实验评价方法,分析了中药在斑马鱼为模型的OP治疗中的研究现状,总结中药抗OP的作用机制及其所涉及的信号通路,对各种斑马鱼OP的造模方法进行了比较和概括,比较了斑马鱼较传统动物模型的优劣,以期为斑马鱼模型在骨相关疾病中的研究提供评价方法借鉴,也为中药抗OP的作用机制相关研究及新药研发提供参考。Osteoporosis(OP)is a systemic metabolic disease with a strong correlation with age.The prevalence of osteoporosis is rising annually as a consequence of the growing issue of population ageing.The current treatments for OP have numerous shortcomings.In contrast,traditional Chinese medicine has a long history and a rich species diversity.Furthermore,recent years have seen an increase in the number of studies examining the anti-OP properties of traditional Chinese medicine.This may provide a safe and effective alternative strategy for the treatment of OP.The zebrafish,due to its favourable optical transparency and high homology with human genes,has been extensively employed as an animal research model in the investigation of human skeletal-related disease mechanisms and drug screening.This paper presents a review of anti-osteoporosis studies of traditional Chinese medicine using zebrafish as a model for osteoporosis.It also provides a summary of the experimental evaluation methods involved in such studies,an analysis of the current status of traditional Chinese medicine in the treatment of osteoporosis using zebrafish as a model,and a summary of the mechanism of action and the signalling pathways involved in traditional Chinese medicine in the anti-osteoporosis treatment of zebrafish.The current research status of Chinese medicine in the treatment of OP was analysed,as well as the mechanism of action of Chinese medicine against OP and the signalling pathways involved.Furthermore,the advantages and disadvantages of various zebrafish modelling methods of OP were compared with those of traditional animal models.The objective of this study is to provide a reference for the evaluation method of the zebrafish model in the study of bone-related diseases,as well as for the study of the mechanism of action of traditional Chinese medicine against OP and for the reference of the research and development of new drugs.

关 键 词：骨质疏松症 斑马鱼 中药 信号通路 药物筛选 

分 类 号：R285[医药卫生—中药学] R289[医药卫生—中医学] R274