电针通过调控P2X7表达改善大鼠肛门切口痛  

Electroacupuncture improves anal incision pain in rats by regulating P2X7 expression

作　　者：高洁 陈艳[1] 常海静 张亚玲 陈斌 GAO Jie;CHEN Yan;CHANG Hai-jing;ZHANG Ya-ling;CHEN Bin(Department of Traditional Chinese Medicine,The First People′s Hospital of Nantong,Nantong 226001;College of Acupuncture and Massage Health and Rehabilitation,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]南通市第一人民医院中医科,江苏南通226001 [2]南京中医药大学针灸推拿养生康复学院,江苏南京210023

出　　处：《解剖科学进展》2025年第1期33-36,共4页Progress of Anatomical Sciences

基　　金：南通市自然科学基金和社会民生科技计划(MSZ2023074)。

摘　　要：目的 探究电针改善大鼠肛门切口痛的作用机制。方法 将40只SD大鼠随机分为对照组、模型组、电针组和非经非穴针刺组,每组10只。测定大鼠机械痛阈,进行糖水偏好实验;取材背根神经节,ELISA检测背根神经节中TNF-α、IL-1β和IL-6含量;HE染色观察病理改变;RT-qPCR法检测大鼠背根神经节P2X7 mRNA表达水平;Western blot检测大鼠背根神经节P2X7蛋白表达。结果 模型组大鼠机械痛阈值和糖水偏好率下降,而电针治疗提升了机械痛阈值与糖水偏好率。对照组大鼠未见明显炎性细胞浸润;模型组大鼠有大量肌纤维坏死,可见大量炎性细胞浸润;电针组大鼠病变缓解。模型组大鼠炎性因子TNF-α、IL-1β及IL-6水平均显著升高,P2X7的mRNA和蛋白表达水平升高,电针治疗后炎性因子表达减少,P2X7表达减少。结论 电针通过调控P2X7表达改善大鼠肛门切口痛。Objective To explore the mechanism of electroacupuncture in improving anal incision pain in rats.Methods Forty SD rats were randomly divided into Control group,Model group,Electroacupuncture group,and Sham non⁃meridian,non⁃acupuncture group,with 10 rats in each group.The control group was not treated with modelling in addition to routine examination,and the remaining groups of rats were established with anal incision pain model.The rats in the electroacupuncture group were given electroacupuncture intervention,the control group and the model group were only fixed on the rat plate,and the non⁃acupuncture and non⁃acupuncture needling group was given superficial stimulation of the non⁃acupuncture points,with only superficial insertion at 20 mm beside the main point,without electricity,and the rats were fixed,needled for the same number of days as the electroacupuncture group.Rats were subjected to mechanical pain threshold measurement,glucose preference test.Levels of TNF⁃α,IL⁃1βand IL⁃6 were detected by ELISA in dorsal root ganglia.HE staining was used to observe pathological changes.RT⁃qPCR was used to detect the relative expression of P2X7 mRNA.Western blot was used to detect the expression of P2X7 protein.Results The mechanical pain threshold and sugar preference rate of Model group rats decreased,while electroacupuncture treatment increased the mechanical pain threshold and sugar preference rate.No significant inflammatory cell infiltration was observed in the control group of rats;Model group rats had a large amount of muscle fiber necrosis and infiltration of inflammatory cells;The lesions in the electroacupuncture group of rats were relieved.The level of inflammatory factor TNF⁃α,IL⁃1βand IL⁃6 significantly increased in Model group rats,and the mRNA and protein expression levels of P2X7 increased.After electroacupuncture treatment,the expression of inflammatory factors decreased,and the expression of P2X7 decreased.Conclusion Electroacupuncture alleviated anal incision pain in rat

关 键 词：P2X7 电针 肛门切口痛 炎症反应 

分 类 号：R657.1[医药卫生—外科学]