元宝枫优树和普通树生长及其AM真菌的特性  

Growth and AM Fungal Characteristics of Acer truncatum Plus Tree and Ordinary Tree

作　　者：费昭雪[1] 李晴 赵鑫 杨诺 张博勇[1] 盛敏[1] FEI Zhao-xue;LI Qing;ZHAO Xin;YANG Nuo;ZHANG Bo-yong;SHENG Min(College of Forestry,Northwest Agriculture and Forestry University,Yangling 712100,Shaanxi,China)

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100

出　　处：《林业科学研究》2025年第2期196-206,共11页Forest Research

基　　金：陕西省自然科学基金项目(2021JM-094);陕西省技术创新专项(2020QFY10-01)。

摘　　要：[目的]本研究以陕西省南部的元宝枫林为研究对象,阐明元宝枫优树和普通树生长状况及其AM真菌的特性,为菌根技术在元宝枫产业发展中的应用奠定基础。[方法]本研究基于元宝枫良种选育的性状标准,筛选元宝枫优树和与其相邻的普通树,采集优树和普通树的根和叶,测定其根系AM真菌侵染状况及根和叶养分含量,提取土壤和根系DNA并进行HiSeq测序,构建优树和普通树的AM真菌群落共现网络,分析优树和普通树AM真菌群落结构差异及其原因。[结果](1)元宝枫优树的土壤pH值、根全氮及根全磷含量显著高于普通树,而树高、叶全氮、叶全磷、叶全钾、根际土壤铵态氮、硝态氮、速效磷、速效钾及有机碳含量则显著低于普通树。(2)优树根际AM真菌菌丝密度和Chao指数显著高于普通树。(3)优树根系和根际AM真菌群落共现网络拓扑结构比普通树更为复杂,筛选二者差异OTU发现其主要集中在元宝枫根系。(4)元宝枫根系AM真菌群落多样性与根全磷含量显著相关;土壤pH值、树高和胸径与有机碳、速效钾、速效磷、铵态氮和硝态氮含量呈负相关。[结论]元宝枫优树和普通树根内或根际AM真菌特性差异显著,且这些差异与元宝枫植株养分状况及根际土壤特性关系密切。[Objective]This study investigates the growth performance of Acer truncatum plus trees and ordinary trees,as well as the characteristics of arbuscular mycorrhizal(AM)fungi in a forest located in southern Shaanxi Province.It aims to establish a foundation for the application of mycorrhizal technology in the development of the A.truncatum industry.[Method]Based on the selection criteria of Acer truncatum seed traits,superior trees were identified and compared with their neighboring ordinary trees.Roots and leaves from both the superior trees and the ordinary trees were collected for analysis.The AM fungal infection status of the root systems and the nutrient content of both roots and leaves were determined.Soil and root DNA were extracted,and HiSeq sequencing was performed.A co-occurrence network of the AM fungal communities was constructed,and differences in AM fungal community structure between the plus and ordinary trees were analyzed,along with the underlying causes for these differences.[Results](1)Soil pH value,root total nitrogen and root total phosphorus contents in A.truncatum plus trees were significantly higher than those of ordinary trees.In contrast,tree height,leaf total nitrogen,leaf total phosphorus,leaf total potassium,rhizosphere soil ammonium nitrogen,nitrate nitrogen,available phosphorus,available potassium and organic carbon contents were significantly lower than those of ordinary trees.(2)The interroot AM fungal mycelial density and Chao index of plus trees were significantly higher than those of ordinary trees.(3)The topology of the co-occurrence network of root and inter-root AM fungal communities in plus trees was more complex than that in ordinary trees.OTU analysis revealed that differences were primarily concentrated in the root systems of A.truncatum.(4)The diversity of AM fungal communities in the roots of A.truncatum was significantly correlated with root total phosphorus content.Soil pH,tree height and diameter at breast height were negatively correlated with organic carbon,quick-act

关 键 词：元宝枫 优树 丛枝菌根真菌 群落结构 

分 类 号：S718.81[农业科学—林学]