应用再生相关基因突破小麦遗传转化基因型依赖性难题  

Application of Regenerative-Related Genes to Break Through Genotype Dependency in Wheat Genetic Transformation

作　　者：石磊[1,2] 马斯霜 Shi Lei;Ma Sishuang(Agricultural Biotechnology Research Center,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia 750002;Ningxia key Laboratory of Agricultural Biotechnology,Yinchuan,Ningxia 750002)

机构地区：[1]宁夏农林科学院农业生物技术研究中心,宁夏银川750002 [2]宁夏农业生物技术重点实验室,宁夏银川750002

出　　处：《宁夏农林科技》2025年第3期20-24,共5页Journal of Ningxia Agriculture and Forestry Science and Technology

基　　金：宁夏自然科学基金项目(2022AAC05047、2021AAC03277);2021年第六批自治区青年科技人才托举工程。

摘　　要：小麦是世界重要粮食作物之一,但其遗传转化受到转化效率低和基因型依赖强烈的制约。在转化过程中应用再生相关基因能够显著提高遗传转化效率和再生效率,是突破这一难题的有效途径。通过GRF4-GIF1和TaWOX5的过表达,实现了多个小麦品种遗传转化效率的大幅提高,基本解决了小麦遗传转化过程中基因型依赖性难题,具有重要的理论借鉴和实际应用价值。Wheat is one of the most important cereal crops all around the world,but its genetic transformation is restricted by low transformation efficiency and strong genotype dependence.Application of regeneration-related genes in the transformation process can significantly improve genetic transformation efficiency and regeneration efficiency,and is an effective way to break through this problem.The overexpression of GRF4-GIF1 and TaWOX5 has achieved a significant increase in genetic transformation efficiency in several wheat varieties,and almost overcame the genotype-dependent problem in the process of wheat genetic transformation,which have important theoretical reference and practical application value.

关 键 词：小麦 遗传转化 再生相关基因 

分 类 号：F323.21[经济管理—产业经济]